Department of Plant Sciences, School of Life Science, University of Hyderabad, Hyderabad-500046, India.
Bacterial Discovery laboratory, Centre for Environment, Institute of Science & Technology, Jawaharlal Nehru Technological University Hyderabad, Hyderabad-500085, India.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4619-4625. doi: 10.1099/ijs.0.054155-0. Epub 2013 Aug 5.
Two strains (JC83, JC84(T)) of obligately anaerobic, H2S-producing bacteria were isolated from estuarine sediment samples collected from Gangasagar, West Bengal, India. Cells were Gram-stain-negative, non-motile rods. Both strains were positive for oxidase, negative for catalase, hydrolysed casein, reduced nitrate and utilized citrate. Both strains grew chemoorganoheterotrophically with optimal pH of 7-8 (range 7-10) and at 30 °C (range 25-37 °C). C16 : 1ω7c, C18 : 1ω7c, C16 : 0 and C12 : 0 were the major fatty acids of both strains with minor amounts of C14 : 0, C12 : 0 3-OH and C18 : 0. Polar lipids of both strains included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, phosphatidylcholine, phosphatidylinositol, an unidentified aminolipid (AL2), an unidentified phospholipid (PL2) and an unidentified lipid (L3). MK-6 was the major respiratory quinone. The DNA G+C content of strains JC83 and JC84(T) was 25.0 and 24.6 mol%, respectively. The strains showed DNA reassociation >85 % (86.0±0.5 %) (based on DNA-DNA hybridization). Based on 16S rRNA gene sequence analysis, both strains were identified as belonging to the family Campylobacteraceae of the class Epsilonproteobacteria with Arcobacter marinus CL-S1(T) (95.4 % sequence similarity) as their closest phylogenetic neighbour. On the basis of morphological, physiological and chemotaxonomic characteristics as well as phylogenetic analysis, strains JC83 and JC84(T) are considered to represent a novel species, for which the name Arcobacter anaerophilus sp. nov. is proposed. The type strain is JC84(T) ( = KCTC 15071(T) = MTCC 10956(T) = DSM 24636(T)). An emended description of the genus Arcobacter is provided.
从印度西孟加拉邦冈加斯格尔采集的河口沉积物样本中分离到两株严格厌氧、产 H2S 的细菌（JC83、JC84(T)）。细胞革兰氏染色阴性，无动力杆状。两株菌均氧化酶阳性，接触酶阴性，水解酪蛋白，还原硝酸盐，并利用柠檬酸盐。两株菌均以化能有机异养方式生长，最适 pH 为 7-8（范围 7-10），温度为 30°C（范围 25-37°C）。C16 : 1ω7c、C18 : 1ω7c、C16 :0 和 C12 :0 是两株菌的主要脂肪酸，少量存在 C14 :0、C12 :0 3-OH 和 C18 :0。两株菌的极性脂包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰丝氨酸、磷脂酰胆碱、磷脂酰肌醇、一种未鉴定的氨基脂（AL2）、一种未鉴定的磷脂（PL2）和一种未鉴定的脂（L3）。MK-6 是主要的呼吸醌。菌株 JC83 和 JC84(T) 的 DNA G+C 含量分别为 25.0 和 24.6 mol%。菌株的 DNA 重杂交率>85%（86.0±0.5%）（基于 DNA-DNA 杂交）。根据 16S rRNA 基因序列分析，两株菌均被鉴定为弧菌科 ε 变形菌纲的细菌，与 Arcobacter marinus CL-S1(T)（95.4%序列相似性）关系最近。基于形态学、生理学和化学分类学特征以及系统发育分析，菌株 JC83 和 JC84(T) 被认为代表一个新种，提议将其命名为厌氧弧菌 Arcobacter anaerophilus sp. nov.。模式菌株为 JC84(T)（ = KCTC 15071(T) = MTCC 10956(T) = DSM 24636(T)）。对弧菌属的描述进行了修正。
