Rao Chun-Yan, Fu Lan-Ying, Hu Chang-Lun, Chen Dai-Xing, Gan Tian, Wang Yi-Cheng, Zhao Xiao-Yan
Chun-Yan Rao, Lan-Ying Fu, Dai-Xing Chen, Tian Gan, Xiao-Yan Zhao, Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
World J Gastroenterol. 2015 Apr 21;21(15):4555-63. doi: 10.3748/wjg.v21.i15.4555.
To study the effect of hydrogen sulfide (H2S) on severe acute pancreatitis (SAP) in a rat model.
Sprague-Dawley (SD) rats were administered an intraperitoneal injection of saline containing 20% L-Arg (250 mg/100 g) hourly for over 2 h to induce SAP. The rats were treated with DL-propargylglycine (PAG, 50 mg/kg) or different dosages of NaHS (5 mg/kg, 10 mg/kg, 20 mg/kg or 100 mg/kg). PAG or NaHS was administered 1 h before induction of pancreatitis. Rats were sacrificed 24 h after the last L-Arg injection. Blood and pancreas tissues were collected.
The H2S and cystathionine-γ-lyase mRNA levels in SAP rats were significantly lower than those in the control group, and treatment with PAG further reduced the H2S level. Nevertheless, H2S was significantly increased after NaHS administration compared with the SAP group, and the degree of upregulation was associated with the NaHS dosage. NaHS reduced the levels of plasma amylase, interleukin-6 and myeloperoxidase in pancreatic tissue. NaHS suppressed the degradation of IκBα and the activity of nuclear factor-κB, as well as the phosphorylation of PI3K/AKT.
H2S plays an anti-inflammatory role in SAP in vivo.
在大鼠模型中研究硫化氢（H2S）对重症急性胰腺炎（SAP）的影响。
对Sprague-Dawley（SD）大鼠每小时腹腔注射含20% L-精氨酸（250 mg/100 g）的生理盐水，持续2小时以上以诱导SAP。大鼠用DL-炔丙基甘氨酸（PAG，50 mg/kg）或不同剂量的硫氢化钠（NaHS，5 mg/kg、10 mg/kg、20 mg/kg或100 mg/kg）进行治疗。在诱导胰腺炎前1小时给予PAG或NaHS。在最后一次注射L-精氨酸后24小时处死大鼠，采集血液和胰腺组织。
SAP大鼠的H2S和胱硫醚-γ-裂解酶mRNA水平显著低于对照组，用PAG治疗进一步降低了H2S水平。然而，与SAP组相比，给予NaHS后H2S显著升高，上调程度与NaHS剂量相关。NaHS降低了血浆淀粉酶、白细胞介素-6和胰腺组织中髓过氧化物酶的水平。NaHS抑制了IκBα的降解、核因子-κB的活性以及PI3K/AKT的磷酸化。
H2S在体内对SAP发挥抗炎作用。
