Mu Andre, Moreau John W
Moreau Lab, School of Earth Sciences, Faculty of Science, University of Melbourne Melbourne, VIC, Australia ; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne Melbourne, VIC, Australia.
Moreau Lab, School of Earth Sciences, Faculty of Science, University of Melbourne Melbourne, VIC, Australia.
Front Microbiol. 2015 Apr 9;6:263. doi: 10.3389/fmicb.2015.00263. eCollection 2015.
Our primary research paper (Mu et al., 2014) demonstrated selective changes to a deep subsurface prokaryotic community as a result of CO2 stress. Analyzing geochemical and microbial 16S rRNA gene profiles, we evaluated how in situ prokaryotic communities responded to increased CO2 and the presence of trace organic compounds, and related temporal shifts in phylogeny to changes in metabolic potential. In this focused review, we extend upon our previous discussion to present analysis of taxonomic unit co-occurrence profiles from the same field experiment, to attempt to describe dynamic community behavior within the deep subsurface. Understanding the physiology of the subsurface microbial biosphere, including how key functional groups integrate into the community, will be critical to determining the fate of injected CO2. For example, community-wide network analyses may provide insights to whether microbes cooperatively produce biofilm biomass, and/or biomineralize the CO2, and hence, induce changes to formation porosity or changes in electron flow. Furthermore, we discuss potential impacts to the feasibility of subsurface CO2 storage of selectively enriching for particular metabolic functions (e.g., methanogenesis) as a result of CO2 injection.
我们的主要研究论文（Mu等人，2014年）表明，由于二氧化碳胁迫，深层地下原核生物群落发生了选择性变化。通过分析地球化学和微生物16S rRNA基因图谱，我们评估了原位原核生物群落如何应对二氧化碳增加和微量有机化合物的存在，并将系统发育中的相关时间变化与代谢潜力的变化联系起来。在这篇重点综述中，我们在之前讨论的基础上进行扩展，展示对同一田间试验中分类单元共现图谱的分析，试图描述深层地下动态群落行为。了解地下微生物生物圈的生理学，包括关键功能群如何融入群落，对于确定注入二氧化碳的命运至关重要。例如，全群落网络分析可能会提供有关微生物是否协同产生生物膜生物量和/或使二氧化碳生物矿化，从而导致地层孔隙度变化或电子流变化的见解。此外，我们还讨论了由于注入二氧化碳而选择性富集特定代谢功能（如甲烷生成）对地下二氧化碳储存可行性的潜在影响。
