使用PIMMS（实用插入突变定位系统）进行转座子插入定位

Blanchard Adam M, Leigh James A, Egan Sharon A, Emes Richard D

School of Veterinary Medicine and Science, University of Nottingham Loughborough, UK.

Front Genet. 2015 Apr 9;6:139. doi: 10.3389/fgene.2015.00139. eCollection 2015.

UNLABELLED

The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has been developed for simple conditionally essential genome discovery experiments in bacteria. Capable of using raw sequence data files alongside a FASTA sequence of the reference genome and GFF file, PIMMS will generate a tabulated output of each coding sequence with corresponding mapped insertions accompanied with normalized results enabling streamlined analysis. This allows for a quick assay of the genome to identify conditionally essential genes on a standard desktop computer prioritizing results for further investigation.

AVAILABILITY

The PIMMS script, manual and accompanying test data is freely available at https://github.com/ADAC-UoN/PIMMS.

未标记

PIMMS（实用插入突变映射系统）流程已开发用于细菌中简单的条件必需基因组发现实验。PIMMS能够使用原始序列数据文件以及参考基因组的FASTA序列和GFF文件，将生成每个编码序列的表格输出，以及相应的映射插入，并伴有归一化结果，从而实现简化分析。这使得能够在标准台式计算机上快速检测基因组，以识别条件必需基因，并对结果进行优先级排序以供进一步研究。