Baek Seung-Hwa, Nam In-Jeong, Kwak Hyeong Seob, Kim Ki-Chan, Lee Sang-Han
Department of Food Science & Biotechnology, Graduate School, Kyungpook National University, Daegu 702-701, Korea.
Department of Nano-Science & Technology, Graduate School, Kyungpook National University, Daegu 702-701, Korea.
Int J Mol Sci. 2015 Apr 23;16(5):9217-35. doi: 10.3390/ijms16059217.
The aim of this study was to investigate the effect of ethyl acetate fraction of Euryale ferox seed extracts (Efse-EA) on melanogenesis in immortalized mouse melanocyte cell line, melan-a. Efse-EA showed strong dose-dependent mushroom tyrosinase inhibitory activity. Treatment of melan-a cells with 30 μg/mL Efse-EA produced strong inhibition of cellular tyrosinase and melanin synthesis. Efse-EA significantly reduced the levels of melanogenesis-related proteins, such as tyrosinase, tyrosinase-related proteins 1 and 2, and microphthalmia-associated transcription factor. Because Efse-EA treatment reduced tyrosinase protein levels without changing its mRNA expression, we investigated whether this decrease was related to proteasomal or lysosomal degradation of tyrosinase. We found that chloroquine, a lysosomal proteolysis inhibitor, almost completely abolished both the down-regulation of tyrosinase and the inhibition of melanin synthesis induced by Efse-EA. These results suggested that Efse-EA may contribute to the inhibition of melanogenesis by altering lysosomal degradation of tyrosinase, and that this extract may provide a new cosmetic skin-whitening agent.
本研究旨在探讨芡实种子提取物的乙酸乙酯部位（Efse-EA）对永生化小鼠黑素细胞系melan-a中黑色素生成的影响。Efse-EA表现出强烈的剂量依赖性蘑菇酪氨酸酶抑制活性。用30μg/mL的Efse-EA处理melan-a细胞可强烈抑制细胞酪氨酸酶和黑色素合成。Efse-EA显著降低了黑色素生成相关蛋白的水平，如酪氨酸酶、酪氨酸酶相关蛋白1和2以及小眼畸形相关转录因子。由于Efse-EA处理降低了酪氨酸酶蛋白水平而未改变其mRNA表达，我们研究了这种降低是否与酪氨酸酶的蛋白酶体或溶酶体降解有关。我们发现，溶酶体蛋白水解抑制剂氯喹几乎完全消除了Efse-EA诱导的酪氨酸酶下调和黑色素合成抑制。这些结果表明，Efse-EA可能通过改变酪氨酸酶的溶酶体降解来抑制黑色素生成，并且该提取物可能提供一种新的化妆品美白剂。
