Wang Hui, Luo Xia, Meng Shaoquan, Bei Yongjian, Song Tao, Meng Tao, Li Guifen, Zhang Baowei
Anhui Key Laboratory of Eco-engineering and Bio-technique, School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.
College of Life Science & Technology, Yulin Normal University, Yulin, 537000, Guangxi, China.
PLoS One. 2015 Apr 27;10(4):e0125770. doi: 10.1371/journal.pone.0125770. eCollection 2015.
Ichthyophis bannanicus is the only caecilian species in China. In this study, the phylogeography and population demography of I. bannanicus were explored, based on the mitochondrial DNA genes (cyt b and ND2) and 15 polymorphic microsatellite loci. Altogether 158 individuals were collected from five populations in Yunnan province, Guangxi province, Guangdong province, and Northern Vietnam. Phylogeographical and population structure analysis identified either two groups (Xishuangbanna, Northern Vietnam-Yulin-Yangchun-Deqing) or three groups (Xishuangbanna, Northern Vietnam-Yulin-Yangchun, and Deqing), indicating that the Red River and Pearl River systems may have acted as gene-flow barriers for I. bannanicus. Historical population expansion that happened 15-17 Ka ago was detected for mtDNA data and was possibly triggered by warmer weather after the Last Glacial Maximum. However, the Bayesian simulations of population history based on microsatellite data pinpointed population decline in all populations since 19,123 to 1,029 years ago, demonstrating a significant influence of anthropogenic habitat alteration on I. bannanicus.
版纳鱼螈是中国唯一的蚓螈物种。在本研究中，基于线粒体DNA基因（细胞色素b和ND2）以及15个多态性微卫星位点，对版纳鱼螈的系统地理学和种群动态进行了探究。总共从云南省、广西壮族自治区、广东省以及越南北部的五个种群中收集了158个个体。系统地理学和种群结构分析确定了两个组（西双版纳、越南北部-玉林-阳春-德庆）或三个组（西双版纳、越南北部-玉林-阳春以及德庆），这表明红河和珠江水系可能充当了版纳鱼螈的基因流动障碍。线粒体DNA数据检测到15 - 17千年前发生的历史种群扩张，这可能是由末次盛冰期之后的温暖气候引发的。然而，基于微卫星数据的种群历史贝叶斯模拟指出，自19123年至1029年前所有种群的数量都在下降，这表明人为栖息地改变对版纳鱼螈有重大影响。
