Herczeg Dávid, Palomar Gemma, Zieliński Piotr, van Riemsdijk Isolde, Babik Wiesław, Dankovics Róbert, Halpern Bálint, Cvijanović Milena, Vörös Judit
ELKH-ELTE-MTM Integrative Ecology Research Group Budapest Hungary.
Department of Systematic Zoology and Ecology, Institute of Biology ELTE Eötvös Loránd University Budapest Hungary.
Ecol Evol. 2023 Aug 31;13(9):e10478. doi: 10.1002/ece3.10478. eCollection 2023 Sep.
Species with wide-range distributions usually display high genetic variation. This variation can be partly explained by historical lineages that were temporally isolated from each other and are back into secondary reproductive contact, and partly by local adaptations. The smooth newt () is one of the most widely distributed amphibians species across Eurasia and forms a species complex with a partially overlapping distribution and morphology. In the present study, we explored the population genomic structure of smooth newt lineages in the Carpathian Basin (CB) relying on single-nucleotide polymorphisms. Our dataset included new and previously published data to study the secondary contact zone between lineages in the CB and also tested for the barrier effect of rivers to gene flow between these lineages. We confirmed the presence of the South Lineage distributed in Transdanubia and we provided new distribution records of inhabiting the eastern territories of the CB. High genetic diversity of smooth newts was observed, especially in the North Hungarian Mountains and at the interfluves of the main rivers in the South with four distinct lineages of and one lineage of showing a low level of admixture with the spatially closest lineage. Moreover, admixture detected at the interfluve of the main rivers (i.e. Danube and Tisza) suggested a secondary contact zone in the area. Finally, we found that the river Danube has a very weak effect on population divergence, while the river Tisza is a geographical barrier limiting gene flow between smooth newt lineages. As the range boundaries of and in the CB coincide with the river Tisza, our study underpins the influence of rivers in lineage diversification.
分布范围广泛的物种通常表现出高度的遗传变异。这种变异部分可以由历史谱系来解释，这些谱系在时间上相互隔离，现在又重新进入次生生殖接触状态，部分也可以由局部适应性来解释。光滑蝾螈（Lissotriton vulgaris）是欧亚大陆分布最广泛的两栖动物物种之一，形成了一个分布和形态部分重叠的物种复合体。在本研究中，我们依靠单核苷酸多态性探索了喀尔巴阡盆地（CB）光滑蝾螈谱系的种群基因组结构。我们的数据集包括新的和先前发表的数据，用于研究CB中各谱系之间的次生接触带，还测试了河流对这些谱系间基因流动的屏障效应。我们证实了分布在多瑙河以南地区的南线谱系的存在，并提供了栖息在CB东部地区的L. vulgaris的新分布记录。观察到光滑蝾螈具有高度的遗传多样性，特别是在匈牙利北部山区以及南部主要河流的河间地区，有四个不同的L. vulgaris谱系和一个L. montandoni谱系，它们与空间上最接近的L. vulgaris谱系的混合程度较低。此外，在主要河流（即多瑙河和蒂萨河）的河间地区检测到的混合现象表明该地区存在一个次生接触带。最后，我们发现多瑙河对种群分化的影响非常微弱，而蒂萨河是一个限制光滑蝾螈谱系间基因流动的地理屏障。由于CB中L. vulgaris和L. montandoni的分布边界与蒂萨河重合，我们的研究证实了河流在谱系多样化中的影响。
