Imbs Andrey B, Dang Ly P T, Rybin Viacheslav G, Svetashev Vasily I
A.V. Zhirmunsky Institute of Marine Biology, Far-Eastern Branch of the Russian Academy of Sciences, 17 Palchevskogo str., Vladivostok, 690041, Russian Federation,
Lipids. 2015 Jun;50(6):575-89. doi: 10.1007/s11745-015-4021-0. Epub 2015 Apr 28.
The soft corals of the genus Xenia are common for Indo-Pacific reef ecosystems. Lipid class, fatty acid (FA), phospho- and phosphonolipid molecular species compositions were identified for the first time in the soft coral Xenia sp. from Vietnam. Total lipids consisted predominantly of waxes, monoalkyl diacylglycerols, triacylglycerols, sterols, and polar lipids (21.4, 7.7, 14.2, 10.5, and 36.7 %, respectively). Sesquiterpene alcohol, valerenenol, was found. Acids 16:0, 18:3n-6, 20:4n-6, and 20:5n-3 dominated in total FA. The markers of zooxanthellae (18:4n-3 and 18:5n-3) and octocorals (24:5n-6 and 24:6n-3) were detected. Acids 18:5n-3, 20:4n-6, 22:4n-6, and 24:5n-6 concentrated in FA of polar lipids, whereas 14:0, 16:0, 16:1n-7, 18:2n-6, and 18:3n-6 were the major FA of neutral lipids. ChoGpl, EtnGpl, SerGpl, CAEP, PtdIns, and lyso ChoGpl constituted 39.5, 20.8, 20.5, 9.7, 4.3, and 5.3 %, respectively, of the sum of phospho- and phosphonolipids. Thirty-two molecular species of phospholipids and ceramide aminoethylphosphonate (CAEP) were determined by high resolution tandem mass spectrometry. Lyso 18:0e PakCho (4.1 %), 18:0e/20:4 PakCho (20.5 %), 18:1e/20:4 PlsEtn (18.0 %), 18:0e/24:5 PakSer (14.0 %), and 16:0 CAEP (9.6 %) were the major molecular species. EtnGpl and PtdIns mainly consisted of alkenyl acyl and diacyl forms, respectively. Alkyl acyl forms predominated in ChoGpl and SerGpl. Acid 24:5n-6 was a principal FA in SerGpl, whereas 20:4n-6 was more abundant in ChoGpl and EtnGpl. PtdIns contained various C20-24 PUFA. In the context of chemotaxonomy of corals, Xenia sp. has the lipid composition typical for soft corals and the FA profile similar to that of alcyonarians with the high level of 18:3n-6.
Xenia属的软珊瑚在印度 - 太平洋珊瑚礁生态系统中很常见。首次对来自越南的软珊瑚Xenia sp. 的脂质类别、脂肪酸（FA）、磷酸和磷脂分子种类组成进行了鉴定。总脂质主要由蜡、单烷基二酰甘油、三酰甘油、甾醇和极性脂质组成（分别为21.4%、7.7%、14.2%、10.5%和36.7%）。发现了倍半萜醇缬草烯醇。在总脂肪酸中，16:0、18:3n - 6、20:4n - 6和20:5n - 3这几种酸占主导。检测到了虫黄藻的标志物（18:4n - 3和18:5n - 3）以及八放珊瑚的标志物（24:5n - 6和24:6n - 3）。18:5n - 3、20:4n - 6、22:4n - 6和24:5n - 6这些酸集中在极性脂质的脂肪酸中，而14:0、16:0、16:1n - 7、18:2n - 6和18:3n - 6是中性脂质的主要脂肪酸。胆碱甘油磷脂（ChoGpl）、乙醇胺甘油磷脂（EtnGpl）、丝氨酸甘油磷脂（SerGpl）、氨基乙基磷酸神经酰胺（CAEP）、磷脂酰肌醇（PtdIns）和溶血胆碱甘油磷脂（lyso ChoGpl）分别占磷酸和磷脂总和的39.5%、20.8%、20.5%、9.7%、4.3%和5.3%。通过高分辨率串联质谱法测定了32种磷脂和氨基乙基磷酸神经酰胺（CAEP）的分子种类。溶血18:0e磷酸胆碱（4.1%）、18:0e/20:4磷酸胆碱（20.5%）、18:1e/20:4磷脂酰乙醇胺（18.0%）、18:0e/24:5磷酸丝氨酸（14.0%）和16:0 CAEP（9.6%）是主要的分子种类。EtnGpl主要由烯基酰基形式组成，而PtdIns主要由二酰基形式组成。烷基酰基形式在ChoGpl和SerGpl中占主导。24:5n - 6酸是SerGpl中的主要脂肪酸，而20:4n - 6在ChoGpl和EtnGpl中含量更高。PtdIns含有各种C20 - 24多不饱和脂肪酸。在珊瑚的化学分类学背景下，Xenia sp. 具有软珊瑚典型的脂质组成，其脂肪酸谱与具有高水平18:3n - 6的海鸡冠目动物相似。
