Gertler Christoph, Bargiela Rafael, Mapelli Francesca, Han Xifang, Chen Jianwei, Hai Tran, Amer Ranya A, Mahjoubi Mouna, Malkawi Hanan, Magagnini Mirko, Cherif Ameur, Abdel-Fattah Yasser R, Kalogerakis Nicolas, Daffonchio Daniele, Ferrer Manuel, Golyshin Peter N
School of Biological Sciences, Environment Centre Wales, Bangor University, LL57 2UW, Bangor, Gwynedd, UK.
Friedrich-Loeffler-Institut - Federal research Institute for Animal Health, Institute of Novel and Emerging Diseases, Südufer 10, 17493, Greifswald, Insel Riems, Germany.
Microb Ecol. 2015 Oct;70(3):724-40. doi: 10.1007/s00248-015-0606-7. Epub 2015 Apr 29.
Uric acid is a promising hydrophobic nitrogen source for biostimulation of microbial activities in oil-impacted marine environments. This study investigated metabolic processes and microbial community changes in a series of microcosms using sediment from the Mediterranean and the Red Sea amended with ammonium and uric acid. Respiration, emulsification, ammonium and protein concentration measurements suggested a rapid production of ammonium from uric acid accompanied by the development of microbial communities containing hydrocarbonoclastic bacteria after 3 weeks of incubation. About 80 % of uric acid was converted to ammonium within the first few days of the experiment. Microbial population dynamics were investigated by Ribosomal Intergenic Spacer Analysis and Illumina sequencing as well as by culture-based techniques. Resulting data indicated that strains related to Halomonas spp. converted uric acid into ammonium, which stimulated growth of microbial consortia dominated by Alcanivorax spp. and Pseudomonas spp. Several strains of Halomonas spp. were isolated on uric acid as the sole carbon source showed location specificity. These results point towards a possible role of halomonads in the conversion of uric acid to ammonium utilized by hydrocarbonoclastic bacteria.
尿酸是一种很有前景的疏水性氮源，可用于生物刺激受石油污染的海洋环境中的微生物活动。本研究使用来自地中海和红海的沉积物，添加铵和尿酸，研究了一系列微观世界中的代谢过程和微生物群落变化。呼吸作用、乳化作用、铵和蛋白质浓度测量结果表明，尿酸在孵育3周后迅速产生铵，并伴随着含有烃类分解细菌的微生物群落的发展。在实验的最初几天内，约80%的尿酸转化为铵。通过核糖体基因间隔区分析、Illumina测序以及基于培养的技术研究了微生物种群动态。所得数据表明，与嗜盐单胞菌属相关的菌株将尿酸转化为铵，这刺激了以食烷菌属和假单胞菌属为主的微生物群落的生长。以尿酸作为唯一碳源分离出的几株嗜盐单胞菌属菌株表现出位置特异性。这些结果表明嗜盐单胞菌在将尿酸转化为烃类分解细菌利用的铵的过程中可能发挥作用。
