对在加拿大分离的猪戊型肝炎病毒株的全基因组序列及解旋酶结构域中的V239A替换进行分析。
Analysis of complete genome sequences and a V239A substitution in the helicase domain of swine hepatitis E virus strains isolated in Canada.
作者信息
Ward Pierre, Leblanc Danielle, Houde Alain, Brassard Julie
机构信息
Agriculture and Agri-Food Canada, Food Research and Development Centre, 3600 Casavant Blvd West, Saint-Hyacinthe, QC, J2S 8E3, Canada,
出版信息
Arch Virol. 2015 Jul;160(7):1767-73. doi: 10.1007/s00705-015-2429-8. Epub 2015 Apr 29.
Among Canadian swine HEV strains, only one complete genome sequence has been published so far, and there are no data on the virulence of these strains. A collection of 28 Canadian swine HEV strains was used in this study. After RNA extraction, a portion of ORF2, the 3' end of the helicase domain, and two complete genomes were amplified and sequenced. These two new Canadian complete genomes belonged to two different subtypes and showed 87.5 and 87.7% sequence identity to the Canadian swine HEV strain Arkell. The V239A substitution within the helicase domain, which is associated with increased virulence of genotype 3 HEV, was detected in one Canadian swine HEV strain. However, no human hepatitis E infections have been associated with this strain.
在加拿大猪戊型肝炎病毒（HEV）毒株中，迄今为止仅公布了一个完整的基因组序列，且尚无关于这些毒株毒力的数据。本研究使用了一组28株加拿大猪HEV毒株。提取RNA后，对开放阅读框2（ORF2）的一部分、解旋酶结构域的3'末端以及两个完整基因组进行了扩增和测序。这两个新的加拿大完整基因组属于两个不同的亚型，与加拿大猪HEV毒株阿克尔（Arkell）的序列同一性分别为87.5%和87.7%。在一株加拿大猪HEV毒株中检测到解旋酶结构域内的V239A替换，该替换与3型HEV毒力增加有关。然而，尚未发现该毒株与人类戊型肝炎感染有关。
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