Moura Lucas Alves, Ribeiro Fernanda Vieira, Aiello Talita Bianchi, Duek Eliana Ap De Rezende, Sallum Enilson Antonio, Nociti Junior Francisco Humberto, Casati Márcio Zaffalon, Sallum Antonio Wilson
a Division of Periodontics, Department of Prosthodontics and Periodontics, Piracicaba Dental School , State University of Campinas (UNICAMP) , Av. Limeira 901, Caixa Postal: 052, CEP: 13414-903 Piracicaba , São Paulo , Brazil.
J Biomater Sci Polym Ed. 2015;26(10):573-84. doi: 10.1080/09205063.2015.1045249. Epub 2015 Jun 15.
The aim of this pilot study was to assess the release of locally delivered doxycycline by poly (l-lactide-co-glycolide) (PLGA) microspheres in the periodontal pocket of patients with chronic periodontitis, treated by non-surgical periodontal therapy. Nineteen sites of non-adjacent teeth of four different patients were evaluated. Five milligram of PLGA microspheres loaded with 16 doxycycline hyclate (DOX) was administered per periodontal site. To quantify DOX released into the periodontal pocket, gingival crevicular fluid (GCF) was collected from the sites on days 2, 5, 7, 10, 15, and 20 after DOX application, and high-performance liquid chromatography was performed. Data were statistically assessed by ANOVA/Tukey test. At days 2, 5, and 7, the DOX concentration was stably sustained (23.33 ± 1.38, 23.4 ± 1.82, and 22.75 ± 1.33 μg/mL, respectively), with no significant differences over these assessment times (p > 0.05). At days 10 and 15, a tendency was observed toward a decrease in DOX concentration (21.74 ± 0.91 and 20.53 ± 4.88 μg/mL, respectively), but a significant decrease in GCF drug concentration (19.69 ± 4.70 μg/mL) was observed only on day 20. The DOX delivery system developed demonstrated a successful sustained release after local administration, as an adjunct to non-surgical periodontal therapy.
本前瞻性研究的目的是评估聚（左旋丙交酯-共-乙交酯）（PLGA）微球在慢性牙周炎患者非手术牙周治疗后的牙周袋中局部递送强力霉素的释放情况。对4例不同患者的19个非相邻牙齿位点进行了评估。每个牙周位点给予5毫克负载16毫克盐酸强力霉素（DOX）的PLGA微球。为了量化释放到牙周袋中的DOX，在应用DOX后的第2、5、7、10、15和20天从这些位点收集龈沟液（GCF），并进行高效液相色谱分析。数据通过方差分析/图基检验进行统计学评估。在第2、5和7天，DOX浓度稳定维持（分别为23.33±1.38、23.4±1.82和22.75±1.33μg/mL），在这些评估时间内无显著差异（p>0.05）。在第10和15天，观察到DOX浓度有下降趋势（分别为21.74±0.91和20.53±4.88μg/mL），但仅在第20天观察到GCF药物浓度显著下降（19.69±4.70μg/mL）。所开发的DOX递送系统在局部给药后作为非手术牙周治疗的辅助手段成功实现了持续释放。
