Crespo-Sanjuán Jesús, Calvo-Nieves María D, Aguirre-Gervás Beatriz, Herreros-Rodríguez José, Velayos-Jiménez Benito, Castro-Alija María J, Muñoz-Moreno María F, Sánchez Diego, Zamora-González Nuria, Bajo-Grañeras Raquel, García-Centeno Rosa M, Largo Cabrerizo María E, Bustamante María R, Garrote-Adrados José A
Department of Clinical Laboratory, Clinical Hospital, University of Valladolid, Valladolid, Spain
Department of Clinical Laboratory, Clinical Hospital, University of Valladolid, Valladolid, Spain.
Lab Med. 2015 Spring;46(2):123-35. doi: 10.1309/LMZJJU6BC86WUDHW.
To detect whether signs of oxidative stress appear at early stages of colorectal adenocarcinoma (CRC), particularly in the polyp stage. We also aimed to evaluate the specific entities myeloperoxidase (MPO) and oxidized low-density lipoprotein (oxLDL) as novel markers of oxidation in the plasma of patients with CRC and to study the relationship between oxidative status in plasma and patient survival.
We assayed serum or plasma specimens from healthy control subjects (n = 14), from patients with intestinal polyps (n = 39), and from patients with CRC (n = 128) to calculate the modified oxidative balance score (MOBS) using several serum markers (β-carotene, lycopene, vitamin A, vitamin E, MPO, and oxLDL). We also assayed the levels of C-reactive protein (CRP) and obtained lipid profiles. Finally, we studied the survival of patients in relationship to oxidative status (antioxidants and pro-oxidants) and inflammation markers, and added theses data to the lipid profile for each patient.
Oxidative stress levels increased as disease stage advanced. This increase was detected early in the polyp stage, before polyps progressed to cancer, and could be measured by the increase of such new markers as MPO and oxLDL, the decrease in antioxidants, and the MOBS value. Higher levels of oxidation correlated with lower survival.
The oxidation process, which can cause mutations leading to CRC, begins development in the polyp stage. This process may be detected early by monitoring serum markers such as MPO and oxLDL.
检测氧化应激迹象是否出现在结直肠癌（CRC）的早期阶段，特别是息肉阶段。我们还旨在评估髓过氧化物酶（MPO）和氧化型低密度脂蛋白（oxLDL）这两种特定物质作为CRC患者血浆中氧化新标志物的情况，并研究血浆氧化状态与患者生存之间的关系。
我们检测了健康对照受试者（n = 14）、肠息肉患者（n = 39）和CRC患者（n = 128）的血清或血浆标本，使用几种血清标志物（β - 胡萝卜素、番茄红素、维生素A、维生素E、MPO和oxLDL）计算改良氧化平衡评分（MOBS）。我们还检测了C反应蛋白（CRP）水平并获取了血脂谱。最后，我们研究了患者的生存与氧化状态（抗氧化剂和促氧化剂）及炎症标志物的关系，并将这些数据添加到每位患者的血脂谱中。
氧化应激水平随着疾病阶段的进展而升高。这种升高在息肉阶段早期即可检测到，即在息肉进展为癌症之前，并且可以通过MPO和oxLDL等新标志物的增加、抗氧化剂的减少以及MOBS值来衡量。较高的氧化水平与较低的生存率相关。
可导致导致CRC的突变的氧化过程在息肉阶段就开始发展。通过监测MPO和oxLDL等血清标志物可能早期检测到这一过程。
