†State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.
J Nat Prod. 2015 May 22;78(5):1015-25. doi: 10.1021/np500953e. Epub 2015 Apr 28.
Twelve hydrolyzable tannins were obtained from the twigs of Myricaria bracteata, including two new hellinoyl-type dimers, bracteatinins D1 (1) and D2 (2); a new hellinoyl-type trimer, bracteatinin T1 (3); two known monomers, nilotinin M4 (4) and 1,3-di-O-galloyl-4,6-O-(aS)-hexahydroxydiphenoyl-β-d-glucose (5); six known dimers, tamarixinin A (6), nilotinin D8 (7), hirtellins A (10), B (9), and E (8), and isohirtellin C (11); and a known trimer, hirtellin T3 (12). The structures of the tannins were elucidated by spectroscopic data analysis and comparisons to known tannins. All compounds were evaluated as free radical scavengers using 1,1-diphenyl-2-picrylhydrazyl and hydroxy radicals and compared to the activity of BHT and Trolox. Compound 6 showed a significant anti-inflammatory effect on croton oil-induced ear edema in mice (200 mg/kg, inhibition rate 69.8%) and on collagen-induced arthritis in DBA/1 mice (20 mg/kg, inhibition rate 46.0% at day 57).
从中国柽柳的嫩枝中分离得到 12 个水解单宁，包括 2 个新的海廉酰基二聚体，即柽柳素 D1（1）和 D2（2）；1 个新的海廉酰基三聚体，柽柳素 T1（3）；2 个已知的单体，柽柳素 M4（4）和 1,3-二-O-没食子酰基-4,6-O-（aS）-六氢二苯酰基-β-D-葡萄糖（5）；6 个已知的二聚体，柽柳素 A（6）、柽柳素 D8（7）、异海廉素 C（11）、hirtellins A（10）、B（9）和 E（8）和 hirtellin T3（12）；以及 1 个已知的三聚体。通过光谱数据分析和与已知单宁的比较，阐明了单宁的结构。所有化合物均通过 1,1-二苯基-2-苦基肼基和羟基自由基清除实验进行了自由基清除活性评价，并与 BHT 和 Trolox 的活性进行了比较。化合物 6 对小鼠耳肿胀（200mg/kg，抑制率 69.8%）和 DBA/1 小鼠胶原性关节炎（20mg/kg，第 57 天抑制率 46.0%）具有显著的抗炎作用。
