Wang Xiao-Yue, Song Jing-Yuan, Xie Cai-Xiang, Han Jian-Ping, Chen Shi-Lin
Yao Xue Xue Bao. 2014 Dec;49(12):1650-7.
RNA-Sequencing (RNA-Seq) is a newly-developed method in transcriptome research, it can afford more accurate transcription information and be more quickly by using Next-generation Sequencing (NGS) technology. RNA-Seq has been widely used in various biological fields. Genuine traditional Chinese medicines (TCM), with good quality and therapeutic effect, were always praised highly and used by famous physicians. The geo-herbalism formation of TCM is based on the product of the gene expression at specific space and time. So it has been a research hotspot to analyze the mechanism of biosynthesis through RNA-Seq in the study on the secondary metabolism of medicinal plant. This article mainly illustrates the RNA-Seq and its advantages, it also discusses the potential application in genuine TCM, and it can provide useful information for other researchers.
RNA测序（RNA-Seq）是转录组研究中一种新开发的方法，通过使用新一代测序（NGS）技术，它能够提供更准确的转录信息且速度更快。RNA-Seq已在各种生物学领域中广泛应用。道地中药材质量优良、疗效显著，一直备受历代名医推崇和应用。中药的道地性形成是特定时空基因表达的产物。因此，通过RNA-Seq分析药用植物次生代谢的生物合成机制一直是研究热点。本文主要阐述了RNA-Seq及其优势，还探讨了其在道地中药方面的潜在应用，可为其他研究者提供有用信息。
