Flister Michael J, Prokop Jeremy W, Lazar Jozef, Shimoyama Mary, Dwinell Melinda, Geurts Aron
Human and Molecular Genetics Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, 53226, WI, USA,
J Cardiovasc Transl Res. 2015 Jun;8(4):269-77. doi: 10.1007/s12265-015-9626-4. Epub 2015 Apr 29.
The rat has long been a key physiological model for cardiovascular research, most of the inbred strains having been previously selected for susceptibility or resistance to various cardiovascular diseases (CVD). These CVD rat models offer a physiologically relevant background on which candidates of human CVD can be tested in a more clinically translatable experimental setting. However, a diverse toolbox for genetically modifying the rat genome to test molecular mechanisms has only recently become available. Here, we provide a high-level description of several strategies for developing genetically modified rat models of CVD.
长期以来，大鼠一直是心血管研究的关键生理模型，大多数近交系此前已根据对各种心血管疾病（CVD）的易感性或抗性进行了选择。这些CVD大鼠模型提供了一个生理相关背景，在此背景下，可以在更具临床可转化性的实验环境中测试人类CVD的候选因素。然而，用于对大鼠基因组进行基因改造以测试分子机制的多样化工具箱直到最近才出现。在这里，我们对开发CVD基因改造大鼠模型的几种策略进行了高层次描述。
