Carugo Dario, Owen Joshua, Crake Calum, Lee Jeong Yu, Stride Eleanor
Department of Engineering Science, Institute of Biomedical Engineering, University of Oxford, Oxford, United Kingdom.
Department of Engineering Science, Institute of Biomedical Engineering, University of Oxford, Oxford, United Kingdom.
Ultrasound Med Biol. 2015 Jul;41(7):1927-37. doi: 10.1016/j.ultrasmedbio.2015.03.020. Epub 2015 Apr 25.
Ultrasound (US), in combination with microbubbles, has been found to be a potential alternative to viral therapies for transfecting biological cells. The translation of this technique to the clinical environment, however, requires robust and systematic optimization of the acoustic parameters needed to achieve a desired therapeutic effect. Currently, a variety of different devices have been developed to transfect cells in vitro, resulting in a lack of standardized experimental conditions and difficulty in comparing results from different laboratories. To overcome this limitation, we propose an easy-to-fabricate and cost-effective device for application in US-mediated delivery of therapeutic compounds. It comprises a commercially available cell culture dish coupled with a silicon-based "lid" developed in-house that enables the device to be immersed in a water bath for US exposure. Described here are the design of the device, characterization of the sound field and fluid dynamics inside the chamber and an example protocol for a therapeutic delivery experiment.
超声（US）与微泡相结合，已被发现是一种用于转染生物细胞的潜在替代病毒疗法。然而，将该技术转化到临床环境中，需要对实现预期治疗效果所需的声学参数进行稳健且系统的优化。目前，已开发出各种不同的设备用于体外转染细胞，这导致缺乏标准化的实验条件，且不同实验室之间难以比较结果。为克服这一局限性，我们提出一种易于制造且经济高效的设备，用于超声介导的治疗性化合物递送。它包括一个市售的细胞培养皿，以及一个内部开发的硅基“盖子”，该盖子使设备能够浸入水浴中进行超声照射。这里描述了该设备的设计、腔室内声场和流体动力学的表征以及一个治疗性递送实验的示例方案。
