White J W, Winters K
Honeydata Corp., Navasota TX 77868.
J Assoc Off Anal Chem. 1989 Nov-Dec;72(6):907-11.
Using the difference in stable carbon isotope ratio between a honey and its protein fraction permits objective evaluation of possible adulteration of honey with small amounts (7-20%) as well as larger amounts of corn or cane sugar. The present uncertainty in interpretation of results from pure honey with delta 13C values outside the generally accepted limits for pure honey of -27.5% to -23.5% is eliminated; likewise TLC testing to resolve questionable samples with delta 13C values between -23.5 and -21.5% is not needed. Fifty certified samples of pure honey were used to establish criteria for purity, and 38 other samples with delta 13C values in the "questionable" or "adulterated" range for the AOAC official method were tested. A difference of 1.0% or more between honey and protein fractions is proposed to indicate adulteration.
利用蜂蜜与其蛋白质部分稳定碳同位素比率的差异，能够客观评估蜂蜜是否可能被少量（7 - 20%）以及大量的玉米或蔗糖掺假。纯蜂蜜的δ13C值超出纯蜂蜜普遍接受的-27.5%至-23.5%范围时，目前结果解释中的不确定性得以消除；同样，也无需进行薄层层析测试来分辨δ13C值在-23.5%至-21.5%之间的可疑样品。使用50个经认证的纯蜂蜜样品来建立纯度标准，并对另外38个AOAC官方方法中δ13C值处于“可疑”或“掺假”范围的样品进行了测试。提议蜂蜜与其蛋白质部分之间的差异达到1.0%或更大时，表明存在掺假情况。
