Zavaleta Cristina L, Smith Bryan R, Walton Ian, Doering William, Davis Glenn, Shojaei Borzoyeh, Natan Michael J, Gambhir Sanjiv S
Molecular Imaging Program, Department of Radiology and Bio-X Program, Stanford University School of Medicine, Stanford, CA 94305, USA.
Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13511-6. doi: 10.1073/pnas.0813327106. Epub 2009 Jul 28.
Raman spectroscopy is a newly developed, noninvasive preclinical imaging technique that offers picomolar sensitivity and multiplexing capabilities to the field of molecular imaging. In this study, we demonstrate the ability of Raman spectroscopy to separate the spectral fingerprints of up to 10 different types of surface enhanced Raman scattering (SERS) nanoparticles in a living mouse after s.c. injection. Based on these spectral results, we simultaneously injected the five most intense and spectrally unique SERS nanoparticles i.v. to image their natural accumulation in the liver. All five types of SERS nanoparticles were successfully identified and spectrally separated using our optimized noninvasive Raman imaging system. In addition, we were able to linearly correlate Raman signal with SERS concentration after injecting four spectrally unique SERS nanoparticles either s.c. (R(2) = 0.998) or i.v. (R(2) = 0.992). These results show great potential for multiplexed imaging in living subjects in cases in which several targeted SERS probes could offer better detection of multiple biomarkers associated with a specific disease.
拉曼光谱是一种新开发的非侵入性临床前成像技术，为分子成像领域提供了皮摩尔级的灵敏度和多重检测能力。在本研究中，我们展示了拉曼光谱在皮下注射后，能够在活体小鼠体内分离多达10种不同类型的表面增强拉曼散射（SERS）纳米颗粒的光谱指纹。基于这些光谱结果，我们通过静脉注射同时注入了五种最强且光谱独特的SERS纳米颗粒，以成像它们在肝脏中的自然积累情况。使用我们优化的非侵入性拉曼成像系统，成功识别并光谱分离了所有五种类型的SERS纳米颗粒。此外，在皮下（R² = 0.998）或静脉注射（R² = 0.992）四种光谱独特的SERS纳米颗粒后，我们能够将拉曼信号与SERS浓度进行线性关联。这些结果表明，在几种靶向SERS探针能够更好地检测与特定疾病相关的多种生物标志物的情况下，多重成像在活体受试者中具有巨大潜力。
