Ibupoto Z H, Khun K, Liu X, Willander M
J Nanosci Nanotechnol. 2014 Sep;14(9):6704-10. doi: 10.1166/jnn.2014.9374.
The notch signalling plays a vital and radical role for the activity of cellular proliferation, differentiation and apoptosis. In this study, for the first time a particular biosensor is developed for the detection of notch 3. ZnO nanorods were fabricated on the gold coated glass substrate by hydrothermal method and afterwards were decorated with the gold nanoparticles by electrodepositing technique. Scanning electron microscopy (SEM) has shown the perpendicular to the substrate growth pattern of ZnO nanorods. X-ray diffraction (XRD) studies showed the c-axis oriented growth direction with wurtzite crystal structure of ZnO nanorods. X-ray Photoelectron Spectroscopy (XPS) and energy dispersive X-ray (EDX) techniques have shown the presence of Zn, O and Au atoms in the prepared functional material. Furthermore, the anti-notch 3 was physically adsorbed on the gold nanoparticles functionalized ZnO nanorods. The developed potentiometric immunosensor has shown response to the wide range of notch 3 molecules. The detected range included 1.00 x 10(-5)-1.50 x 10(0 ) μg/mL with a sensitivity of 23.15 ± 0.31 mV/decade. The analytical parameters including reproducibility, stability, and selectivity were also investigated and the observed results indicate the acceptable performance of the notch 3 biosensor. Moreover, the proposed notch 3 biosensor exhibited a fast response time of 10 s.
Notch信号通路在细胞增殖、分化和凋亡活动中起着至关重要且根本性的作用。在本研究中，首次开发了一种用于检测Notch 3的特定生物传感器。通过水热法在涂金玻璃基板上制备了ZnO纳米棒，随后采用电沉积技术用金纳米颗粒对其进行修饰。扫描电子显微镜（SEM）显示了ZnO纳米棒垂直于基板的生长模式。X射线衍射（XRD）研究表明ZnO纳米棒具有纤锌矿晶体结构且沿c轴方向生长。X射线光电子能谱（XPS）和能量色散X射线（EDX）技术表明在所制备的功能材料中存在Zn、O和Au原子。此外，抗Notch 3被物理吸附在金纳米颗粒功能化的ZnO纳米棒上。所开发的电位型免疫传感器对广泛范围的Notch 3分子表现出响应。检测范围为1.00×10⁻⁵ - 1.50×10⁰ μg/mL，灵敏度为23.15±0.31 mV/十倍浓度变化。还研究了包括重现性、稳定性和选择性在内的分析参数，观察结果表明Notch 3生物传感器具有可接受的性能。此外，所提出的Notch 3生物传感器表现出10 s的快速响应时间。
