Sivakumar Shivaranjhany, Gupta Archana A, Nik Mohd Rosdy Nik Mohd Mazuan, Venkiteswaran Annapurny, Raj A Thirumal, Awan Kamran Habib
Faculty of Dentistry, Mahsa University, Bandar Saujana Putra Campus, Ministry of Higher Education, Selangor, Malaysia.
Department of Oral Pathology and Microbiology, Dr. D.Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Pune, India.
Transl Cancer Res. 2020 Apr;9(4):3092-3100. doi: 10.21037/tcr.2020.01.09.
This study aims to qualitatively and quantitatively review the association between Epstein-Barr virus (EBV) and oral squamous cell carcinoma (OSCC).
PubMed, Scopus, and Web of Science databases were searched using the keywords "EBV or Epstein Barr virus and Oral cancer or Oral squamous cell carcinoma" for published case-control studies in the English language upto August 2019.
The search yielded 985 articles out of which 966 articles were excluded by screening their titles and abstracts as they were irrelevant or duplicates. Based on the full-text assessment of the remaining 19 articles, only 7 satisfied the inclusion criteria and were included in the qualitative analysis, out of which only 4 were compatible to be included in the meta-analysis. The diagnostic modalities used included immunohistochemistry, hybridization and polymerase chain reaction. The diagnostic targets included latent membrane protein (LMP)-1, EBV determined nuclear antigen-1, EBV-encoded small non-polyadenylated RNA-2. The meta-analysis showed that there is an association between the EBV and OSCC.
Determining the association of EBV with OSCC is highly tedious due to the contrasting data obtained from individuals' studies which in turn is due to the wide variations in the sensitivity and specificity of the diagnostic modalities used and diagnostic targets selected. Although the meta-analysis revealed an association between EBV and OSCC, the number and the quality of the studies included in the meta-analysis are limited, thus the association requires further validation for any conclusive inference.
本研究旨在对爱泼斯坦-巴尔病毒（EBV）与口腔鳞状细胞癌（OSCC）之间的关联进行定性和定量综述。
在PubMed、Scopus和Web of Science数据库中检索截至2019年8月发表的英文病例对照研究，关键词为“EBV或爱泼斯坦-巴尔病毒与口腔癌或口腔鳞状细胞癌”。
检索共得到985篇文章，通过筛选标题和摘要，排除了966篇不相关或重复的文章。基于对其余19篇文章的全文评估，只有7篇符合纳入标准并纳入定性分析，其中只有4篇适合纳入荟萃分析。使用的诊断方法包括免疫组织化学、杂交和聚合酶链反应。诊断靶点包括潜伏膜蛋白（LMP）-1、EBV核抗原-1、EBV编码的小非聚腺苷酸化RNA-2。荟萃分析表明EBV与OSCC之间存在关联。
由于个体研究获得的数据相互矛盾，确定EBV与OSCC的关联非常繁琐，这反过来又归因于所用诊断方法和所选诊断靶点的敏感性和特异性差异很大。尽管荟萃分析显示EBV与OSCC之间存在关联，但荟萃分析中纳入的研究数量和质量有限，因此该关联需要进一步验证才能得出任何确定性结论。
