Aldo-Benson M, Brooks M S, Scheiderer-Pratt L
Department of Medicine, Indiana University School of Medicine, Indianapolis.
Immunol Res. 1989;8(4):271-80. doi: 10.1007/BF02935512.
Generalized increase in immunoglobulin secretion, which is a prominent feature of autoimmune diseases, may be due to abnormal T cell regulation, intrinsic abnormality of B cells, or both. To investigate this question we developed nonmalignant continuous B lymphocyte lines from 20-week-old BWF1 mice and compared their growth and immune response to that of BALB/c mice cell lines. The B cell lines contain less than 1% T cells and macrophages and require growth factors from phytohemagglutinin-stimulated EL-4 lymphoma (GF) or recombinant interleukin 4 for continuous growth. No antigens or mitogens are required for growth. In the presence of 20% GF (which is optimal for BALB/c cell growth and immune function) spontaneous growth of BWF1 B cells, and spontaneous entry into G1, was similar to that of BALB/c B cells. With concentrations of GF and anti-mu which were optimal for BALB/c, the growth and immune response of isolated BWF1 B cells are no different from those of BALB/c controls, but at suboptimal doses of GF there is a significant increase of both spontaneous immunoglobulin secretion and response to anti-mu in BWF1 B cells. Thus, these autoimmune B cells are more sensitive to the effects of both T cell factors and immunoglobulin receptors stimulation.
免疫球蛋白分泌普遍增加是自身免疫性疾病的一个显著特征，这可能是由于T细胞调节异常、B细胞内在异常或两者兼而有之。为了研究这个问题，我们从20周龄的BWF1小鼠中建立了非恶性连续B淋巴细胞系，并将它们的生长和免疫反应与BALB/c小鼠细胞系进行了比较。这些B细胞系含有不到1%的T细胞和巨噬细胞，需要来自植物血凝素刺激的EL-4淋巴瘤的生长因子（GF）或重组白细胞介素4才能持续生长。生长不需要抗原或有丝分裂原。在存在20% GF（这对BALB/c细胞生长和免疫功能是最佳的）的情况下，BWF1 B细胞的自发生长以及自发进入G1期的情况与BALB/c B细胞相似。在对BALB/c最佳的GF和抗μ浓度下，分离的BWF1 B细胞的生长和免疫反应与BALB/c对照无异，但在GF剂量次优时，BWF1 B细胞的自发免疫球蛋白分泌和对抗μ的反应均显著增加。因此，这些自身免疫性B细胞对T细胞因子和免疫球蛋白受体刺激的作用更敏感。
