Elbe H, Dogan Z, Taslidere E, Cetin A, Turkoz Y
Department of Histology and Embryology, Faculty of Medicine, Mugla Sıtkı Kocman University, Mugla, Turkey
Department of Anatomy, Faculty of Medicine, Adiyaman University, Adiyaman, Turkey.
Hum Exp Toxicol. 2016 Mar;35(3):276-81. doi: 10.1177/0960327115584686. Epub 2015 Apr 30.
Ciprofloxacin is a broad-spectrum quinolone antibiotic commonly used in clinical practice. Quercetin is an antioxidant belongs to flavonoid group. It inhibits the production of superoxide anion. In this study, we aimed to evaluate the effects of quercetin on renal injury and oxidative stress caused by ciprofloxacin. Twenty-eight female Wistar albino rats were divided into four groups: control, quercetin (20 mg kg(-1) day(-1) gavage for 21 days), ciprofloxacin (20 mg kg(-1) twice a day intraperitoneally for 10 days), and ciprofloxacin + quercetin. Samples were processed for histological and biochemical evaluations. Malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD), and catalase (CAT) activities were measured in kidney tissue. The ciprofloxacin group showed histopathological changes such as infiltration, dilatation in tubules, tubular atrophy, reduction of Bowman's space, congestion, hemorrhage, and necrosis. In the ciprofloxacin + quercetin group, these histopathological changes markedly reduced. MDA levels increased in the ciprofloxacin group and decreased in the ciptofloxacin + quercetin group. SOD and CAT activities and GSH levels significantly decreased in the ciprofloxacin group. On the other hand, in the ciprofloxacin + quercetin group, SOD and CAT activities and GSH levels significantly increased with regard to the ciprofloxacin group. We concluded that quercetin has antioxidative and therapeutic effects on renal injury and oxidative stress caused by ciprofloxacin in rats.
环丙沙星是临床实践中常用的一种广谱喹诺酮类抗生素。槲皮素是一种属于黄酮类的抗氧化剂。它可抑制超氧阴离子的产生。在本研究中，我们旨在评估槲皮素对环丙沙星所致肾损伤和氧化应激的影响。将28只雌性Wistar白化大鼠分为四组：对照组、槲皮素组（20 mg kg⁻¹ 每日灌胃，共21天）、环丙沙星组（20 mg kg⁻¹ 每日腹腔注射两次，共10天）以及环丙沙星 + 槲皮素组。对样本进行组织学和生化评估。测定肾组织中的丙二醛（MDA）和谷胱甘肽（GSH）水平、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性。环丙沙星组出现组织病理学变化，如浸润、肾小管扩张、肾小管萎缩、鲍曼间隙减小、充血、出血和坏死。在环丙沙星 + 槲皮素组中，这些组织病理学变化明显减轻。环丙沙星组MDA水平升高，而环丙沙星 + 槲皮素组MDA水平降低。环丙沙星组SOD和CAT活性以及GSH水平显著降低。另一方面，与环丙沙星组相比，环丙沙星 + 槲皮素组SOD和CAT活性以及GSH水平显著升高。我们得出结论，槲皮素对大鼠环丙沙星所致肾损伤和氧化应激具有抗氧化和治疗作用。
