Dogan Z, Elbe H, Taslidere E, Soysal H, Cetin A, Demirtas S
a Department of Anatomy, Faculty of Medicine , Adiyaman University , Adiyaman.
b Department of Histology and Embryology, Faculty of Medicine , Mugla Sıtkı Kocman University , Mugla.
Biotech Histochem. 2017;92(7):481-486. doi: 10.1080/10520295.2017.1356469. Epub 2017 Aug 24.
Urinary tract infections are common in pregnant women and ciprofloxacin frequently is used as a broad spectrum antibiotic. It has been suggested that ciprofloxacin causes liver damage in fetuses. Quercetin is a flavonoid with antioxidant properties. We investigated the efficacy of quercetin treatment for preventing fetal liver damage caused by ciprofloxacin. Pregnant rats were divided into four groups: untreated control group (C), 20 mg/kg quercetin for 21 days group (Q), 20 mg/kg twice/day ciprofloxacin for 10 days group (CP), and 20 mg/kg, ciprofloxacin + quercetin for 21 days group (CP + Q). Fetal livers were removed on day 21 of gestation to measure antioxidants and for histological observation. Malondialdehyde (MDA) and glutathione (GSH) levels, and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities were measured in tissue samples. GSH-Px, SOD and CAT activities were significantly lower in the CP group compared to group C. A significant increase in MDA was observed in the CP group compared to group C. There was no significant difference in GSH levels in any group. MDA levels were lower and CAT, SOD and GSH-Px enzyme activities were higher in the CP + Q group compared to group CP. Liver samples of the CP group exhibited central vein dilation, portal vein congestion, pyknotic nuclei and cytoplasmic vacuolization in some hepatocytes. Histological changes were less prominent in the rats treated with quercetin. Use of ciprofloxacin during pregnancy caused oxidative damage in fetal liver tissue. Oxidative stress was ameliorated by quercetin. Quercetin supports the antioxidant defense mechanism and it is beneficial for treating fetal liver damage caused by ciprofloxacin.
尿路感染在孕妇中很常见，环丙沙星常被用作广谱抗生素。有人认为环丙沙星会导致胎儿肝脏损伤。槲皮素是一种具有抗氧化特性的类黄酮。我们研究了槲皮素治疗对预防环丙沙星所致胎儿肝脏损伤的疗效。将怀孕大鼠分为四组：未治疗对照组（C）、20mg/kg槲皮素治疗21天组（Q）、20mg/kg环丙沙星每日两次共治疗10天组（CP）以及20mg/kg环丙沙星+槲皮素治疗21天组（CP+Q）。在妊娠第21天取出胎儿肝脏，用于测量抗氧化剂水平并进行组织学观察。测定组织样本中的丙二醛（MDA）和谷胱甘肽（GSH）水平，以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）活性。与C组相比，CP组的GSH-Px、SOD和CAT活性显著降低。与C组相比，CP组的MDA显著增加。各组的GSH水平无显著差异。与CP组相比，CP+Q组的MDA水平较低，CAT、SOD和GSH-Px酶活性较高。CP组的肝脏样本显示中央静脉扩张、门静脉淤血、一些肝细胞出现核固缩和细胞质空泡化。在接受槲皮素治疗的大鼠中，组织学变化不那么明显。孕期使用环丙沙星会导致胎儿肝脏组织发生氧化损伤。槲皮素可减轻氧化应激。槲皮素支持抗氧化防御机制，对治疗环丙沙星所致胎儿肝脏损伤有益。
