豚鼠结肠带肌细胞钙激活钾通道的通透、选择性和阻断作用
Permeation, selectivity, and blockade of the Ca2+-activated potassium channel of the guinea pig taenia coli myocyte.
作者信息
Hu S L, Yamamoto Y, Kao C Y
机构信息
Department of Pharmacology, State University of New York, Brooklyn 11203.
出版信息
J Gen Physiol. 1989 Nov;94(5):849-62. doi: 10.1085/jgp.94.5.849.
Abstract
The permeation properties of the 147-pS Ca2+-activated K+ channel of the taenia coli myocytes are similar to those of the delayed rectifier channel in other excitable membranes. It has a selectivity sequence of K+ 1.0 greater than Rb+ 0.65 greater than NH4+ 0.50. Na+, Cs+, Li+, and TEA+ (tetraethylammonium) are impermeant. Internal Na+ blocks K+ channel in a strongly voltage-dependent manner with an equivalent valence (zd) of 1.20. Blockade by internal Cs+ and TEA+ is less voltage dependent, with d of 0.61 and 0.13, and half-blockage concentrations of 88 and 31 mM, respectively. External TEA+ is about 100 times more effective in blocking the K+ channel. All these findings suggest that the 147-pS Ca2+-activated K+ channel in the taenia myocytes, which functions physiologically like the delayed rectifier, is the single-channel basis of the repolarizing current in an action potential.
摘要
结肠带肌细胞中147-pS钙激活钾通道的通透特性与其他可兴奋膜中的延迟整流通道相似。其选择性顺序为：K⁺（1.0）＞Rb⁺（0.65）＞NH₄⁺（0.50）。Na⁺、Cs⁺、Li⁺和TEA⁺（四乙铵）不通透。细胞内Na⁺以强电压依赖性方式阻断钾通道，等效价（zd）为1.20。细胞内Cs⁺和TEA⁺的阻断对电压的依赖性较小，d分别为0.61和0.13，半阻断浓度分别为88 mM和31 mM。细胞外TEA⁺在阻断钾通道方面的效力约高100倍。所有这些发现表明，结肠带肌细胞中的147-pS钙激活钾通道在生理功能上类似于延迟整流通道，是动作电位复极化电流的单通道基础。
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