Department of Physical Chemistry and Applied Thermodynamics, EPS, Edificio Leonardo da Vinci, Campus de Rabanales, Campus de Excelencia Internacional Agroalimentario ceiA3, 14071 Cordoba, Spain.
Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.
Bioresour Technol. 2015 Aug;190:57-65. doi: 10.1016/j.biortech.2015.03.114. Epub 2015 Mar 31.
This study focuses on the valorisation of crude glycerol and sunflower meal (SFM) from conventional biodiesel production plants for the separation of value-added co-products (antioxidant-rich extracts and protein isolate) and for enhancing biodiesel production through microbial oil synthesis. Microbial oil production was evaluated using three oleaginous yeast strains (Rhodosporidium toruloides, Lipomyces starkeyi and Cryptococcus curvatus) cultivated on crude glycerol and nutrient-rich hydrolysates derived from either whole SFM or SFM fractions that remained after separation of value-added co-products. Fed-batch bioreactor cultures with R. toruloides led to the production of 37.4gL(-1) of total dry weight with a microbial oil content of 51.3% (ww(-1)) when a biorefinery concept based on SFM fractionation was employed. The estimated biodiesel properties conformed with the limits set by the EN 14214 and ASTM D 6751 standards. The estimated cold filter plugging point (7.3-8.6°C) of the lipids produced by R. toruloides is closer to that of biodiesel derived from palm oil.
本研究侧重于从传统生物柴油生产厂中粗甘油和葵花饼（SFM）的增值，以分离附加值的副产品（富含抗氧化剂的提取物和蛋白质分离物），并通过微生物油合成来提高生物柴油的产量。使用三种产油酵母菌株（Rhodosporidium toruloides、Lipomyces starkeyi 和 Cryptococcus curvatus），在粗甘油和源自整个 SFM 或在分离附加值副产品后剩余的 SFM 馏分的营养丰富的水解产物上进行微生物油生产的评估。采用基于 SFM 分级的生物炼制概念，R. toruloides 的分批补料生物反应器培养可生产 37.4g/L 的总干重，微生物油含量为 51.3%（ww(-1)）。估计的生物柴油特性符合 EN 14214 和 ASTM D 6751 标准规定的限制。R. toruloides 生产的油脂的冷滤堵塞点（7.3-8.6°C）估计更接近棕榈油衍生的生物柴油。
