Qi Feng, Zhao Xuebing, Kitahara Yuki, Li Tian, Ou Xianjin, Du Wei, Liu Dehua, Huang Jianzhong
College of Life Sciences Fujian Normal University Fuzhou, Fujian China.
Institute of Applied Chemistry Department of Chemical Engineering Tsinghua University Beijing China.
Eng Life Sci. 2016 Mar 29;17(3):249-261. doi: 10.1002/elsc.201500143. eCollection 2017 Mar.
The oleaginous yeast has been considered as an economical lipid producer because it transforms carbohydrates from lignocellulosic hydrolyzate into triglycerides; however, cannot survive in hydrolyzate due to the inhibitors co-produced by hydrolysis. We have previously reported a plasma mutagenesis-generated mutant strain M18 that had strong tolerance for the stress environments of hydrolyzate. Here, we applied transcriptomic and proteomic approaches to analyze the global metabolic responses to the stress in hydrolyzate of and elucidate the tolerant mechanism of the mutant strain. The results showed that 57% genes matched and correlated well with their corresponding proteins. Five hundred and seven genes and 366 proteins had their transcription and expression levels changed, respectively, and 39 key genes with significantly changed transcription and expression levels (≥5-fold changes) were identified. The results demonstrated that four cellular processes and their key genes are likely related to the mechanism of tolerance of M18 strain. Enhanced expression of the key genes in could improve the cellular stress tolerance to lignocellulosic hydrolyzate, while the altered expression of most key genes is probably not caused by mutagenesis, but induced by stressful environments of the hydrolyzate.
产油酵母被认为是一种经济的脂质生产者，因为它能将木质纤维素水解产物中的碳水化合物转化为甘油三酯；然而，由于水解过程中共同产生的抑制剂，它无法在水解产物中存活。我们之前报道过一株通过等离子体诱变产生的突变菌株M18，它对水解产物的应激环境具有很强的耐受性。在此，我们应用转录组学和蛋白质组学方法分析了M18对水解产物应激的全局代谢反应，并阐明了突变菌株的耐受机制。结果表明，57%的基因与其相应蛋白质匹配良好且相关性高。分别有507个基因和366个蛋白质的转录和表达水平发生了变化，并且鉴定出39个转录和表达水平有显著变化（≥5倍变化）的关键基因。结果表明，四个细胞过程及其关键基因可能与M18菌株的耐受机制有关。增强关键基因在M18中的表达可以提高细胞对木质纤维素水解产物的应激耐受性，而大多数关键基因表达的改变可能不是由诱变引起的，而是由水解产物的应激环境诱导的。
