Kennedy Alan, Spiers J Paul, Crowley Vivion, Williams Emlyn, Lithander Fiona E
Department of Pharmacology and Therapeutics, Trinity College Dublin, Dublin, Ireland.
Department of Pharmacology and Therapeutics, Trinity College Dublin, Dublin, Ireland.
Nutrition. 2015 Jun;31(6):863-70. doi: 10.1016/j.nut.2015.01.009. Epub 2015 Feb 26.
Evidence suggests that an acute systemic inflammatory response is invoked after consumption of a high-energy meal. Postprandial regulation of adiponectin, an adipose tissue-derived, anti-inflammatory hormone, and the gelatinases, matrix metalloproteinase (MMP)-2 and MMP-9, endopeptidases implicated in a diverse range of inflammatory processes, remain inconclusive. The aim of this study was to assess the postprandial effect of a high-energy (1212 kcal) meal on plasma adiponectin, MMP-2 and MMP-9 activity, glucose, insulin, triacylglycerols, total cholesterol, high-density lipoprotein cholesterol, and the differential effects on these parameters depending on whether the test meal was high fat (HF; 46 g fat, 1210 kcal) or isoenergetic and low fat (LF; 15 g fat, 1214 kcal energy).
Test meals were consumed by 17 lean, healthy men on two separate occasions with blood samples collected by venipuncture at baseline (0 h) and 1 and 3 h after consumption of each test meal.
At baseline, no significant difference was seen in the parameters between the two groups, except for MMP-2, MMP-9, and total cholesterol. Over the 3-h postprandial period, no significant differential effect of the HF versus the LF test meal was observed on adiponectin, MMP-2, MMP-9, or on metabolic markers other than triacylglycerol, which increased significantly in response to the HF test meal (time × treatment, P = 0.002). When analyzed independent of time, MMP-2 (treatment, P = 0.006), MMP-9 (treatment, P = 0.022), and glucose (treatment, P = 0.026) were lower after consumption of the HF meal compared with the LF test meal. When analyzed independent of treatment, adiponectin increased over the 3-h postprandial period (time, P = 0.031), but there was no change in MMP-2 or MMP-9 (time, P = 0.503 and P = 0.525, respectively). Over the 3-h postprandial period, insulin (time, P < 0.001) and total cholesterol (time, P = 0.002) increased, whereas glucose (time, P < 0.001) and high-density lipoprotein cholesterol (time, P < 0.001) decreased.
No differential effects of a HF versus a LF isoenergetic meal were seen on postprandial adiponectin or the gelatinases. Adiponectin increased in response to a high-energy meal independent of treatment, and the gelatinases were lower in response to the HF versus the LF isoenergetic meal, independent of time point. Given the considerable amount of time that humans spend in the postprandial state, additional research is necessary to further understand inflammatory changes in this state.
有证据表明，食用高能膳食后会引发急性全身炎症反应。脂联素是一种由脂肪组织分泌的抗炎激素，而明胶酶（基质金属蛋白酶（MMP）-2和MMP-9）是参与多种炎症过程的内肽酶，它们的餐后调节作用尚无定论。本研究的目的是评估高能（1212千卡）膳食对血浆脂联素、MMP-2和MMP-9活性、葡萄糖、胰岛素、三酰甘油、总胆固醇、高密度脂蛋白胆固醇的餐后影响，以及根据试验膳食是高脂肪（HF；46克脂肪，1210千卡）还是等能量低脂（LF；15克脂肪，1214千卡能量）对这些参数的不同影响。
17名健康瘦男子在两个不同的时间点食用试验膳食，并在基线（0小时）以及每次食用试验膳食后1小时和3小时通过静脉穿刺采集血样。
在基线时，除MMP-2、MMP-9和总胆固醇外，两组之间的参数无显著差异。在餐后3小时内，未观察到HF试验膳食与LF试验膳食对脂联素、MMP-2、MMP-9或除三酰甘油以外的代谢标志物有显著的差异影响，三酰甘油对HF试验膳食有显著升高反应（时间×处理，P = 0.002）。独立于时间分析时，与LF试验膳食相比，食用HF膳食后MMP-2（处理，P = 0.006）、MMP-9（处理，P = 0.022）和葡萄糖（处理，P = 0.026）较低。独立于处理分析时，脂联素在餐后3小时内升高（时间，P = 0.031），但MMP-2或MMP-9无变化（时间，P分别为0.503和0.525）。在餐后3小时内，胰岛素（时间，P < 0.001）和总胆固醇（时间，P = 0.002）升高，而葡萄糖（时间，P < 0.001）和高密度脂蛋白胆固醇（时间，P < 0.001）降低。
HF等能量膳食与LF等能量膳食对餐后脂联素或明胶酶无差异影响。无论处理如何，脂联素对高能膳食有反应而升高，并且与LF等能量膳食相比，HF等能量膳食使明胶酶降低，与时间点无关。鉴于人类在餐后状态花费的时间相当长，有必要进行更多研究以进一步了解该状态下的炎症变化。
