State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Western Xianning Rd. 28#, Xi'an 710049, China.
State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Western Xianning Rd. 28#, Xi'an 710049, China.
Carbohydr Polym. 2015 Aug 1;126:192-8. doi: 10.1016/j.carbpol.2015.03.013. Epub 2015 Mar 14.
In this study, in order to better mimick the nature of bone extracellular matrix, hyaluronic acid (HA) hydrogels having a triple degradation behavior were synthesized from 3,3'-dithiodipropionate hydrazide-modified HA (DTPH-HA) and polyethylene glycol dilevulinate (LEV-PEG-LEV) via the reaction of the ketone carbonyl groups of LEV-PEG-LEV with the hydrazide groups of DTPH-HA. The HA hydrogels were characterized by solid state (13)C NMR, FT-IR, SEM, and rheological, swelling and degradation tests. The results showed that the HA hydrogels exhibited a highly porous morphology and had pore diameters ranging from 20 to 200 μm. The equilibrium swelling ratio of the HA hydrogels was no less than 37.5. The HA hydrogels could be degraded by hyaluronidase and reducing substances or at acidic pH values. The biocompatibility of the HA hydrogels was evaluated using osteoblast-like MC3T3-E1 cells by live/dead staining and MTT assays. The results revealed that the HA hydrogels had good biocompatibility and could support the attachment and proliferation of MC3T3-E1 cells. All the results indicated that the HA hydrogels synthesized by hydrazone bond crosslinking might have great potential to be used in bone tissue engineering.
在这项研究中，为了更好地模拟骨细胞外基质的性质，通过将 3,3'-二硫代二丙酰胺基修饰的透明质酸（DTPH-HA）与聚乙烯醇二缩醛（LEV-PEG-LEV）的酮羰基与 DTPH-HA 的酰肼基团反应，合成了具有三重降解行为的透明质酸（HA）水凝胶。通过固态（13）C NMR、FT-IR、SEM 和流变学、溶胀和降解测试对 HA 水凝胶进行了表征。结果表明，HA 水凝胶具有高度多孔的形态，孔径范围为 20 至 200 μm。HA 水凝胶的平衡溶胀比不低于 37.5。HA 水凝胶可以在透明质酸酶和还原物质或酸性 pH 值的作用下降解。通过活/死染色和 MTT 测定评估了 HA 水凝胶对成骨样 MC3T3-E1 细胞的生物相容性。结果表明，HA 水凝胶具有良好的生物相容性，能够支持 MC3T3-E1 细胞的附着和增殖。所有结果表明，通过腙键交联合成的 HA 水凝胶可能具有很大的潜力用于骨组织工程。
