Parker H. Petit Institute for Bioengineering and Bioscience, G.W. Woodruff School of Mechanical Engineering and School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA, USA; Center of Excellence in Biomaterials, Department of Biomedical Engineering and Department of Chemical Engineering, Amirkabir University of Technology, Tehran, Iran; Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Center of Excellence in Biomaterials, Department of Biomedical Engineering and Department of Chemical Engineering, Amirkabir University of Technology, Tehran, Iran.
Nanomedicine. 2015 Oct;11(7):1809-19. doi: 10.1016/j.nano.2015.04.005. Epub 2015 Apr 29.
Advancement of bone tissue engineering as an alternative for bone regeneration has attracted significant interest due to its potential in reducing the costs and surgical trauma affiliated with the effective treatment of bone defects. We have improved the conventional approach of producing polymeric nanoparticles, as one of the most promising choices for drug delivery systems, using a microfluidics platform, thus further improving our control over osteogenic differentiation of mesenchymal stem cells. Molecular dynamics simulations were carried out for theoretical understanding of our experiments in order to get a more detailed molecular-scale insight into the drug-carrier interactions. In this work, with the sustained intracellular delivery of dexamethasone from microfluidics-synthesized nanoparticles, we explored the effects of particle design on controlling stem cell fates. We believe that the insights learned from this work will lead to the discovery of new strategies to tune differentiation for in situ differentiation or stem cell therapeutics.
The use of mesenchymal stem cells has been described by many researchers as a novel therapy for bone regeneration. One major hurdle in this approach is the control of osteogenic differentiation. In this article, the authors described elegantly their microfluidic system in which dexamethasone loaded nanoparticles were produced. This system would allow precise fabrication of nanoparticles and consequently higher efficiency in cellular differentiation.
由于其在降低与骨缺损有效治疗相关的成本和手术创伤方面的潜力，骨组织工程学的发展作为骨再生的一种替代方法引起了极大的兴趣。我们使用微流控平台改进了生产聚合物纳米粒子的传统方法，作为药物输送系统最有前途的选择之一，从而进一步提高了我们对间充质干细胞成骨分化的控制。为了从理论上理解我们的实验，进行了分子动力学模拟，以便更详细地了解药物载体相互作用的分子尺度。在这项工作中，通过从微流控合成的纳米粒子中持续递送入胞内的地塞米松，我们探索了颗粒设计对控制干细胞命运的影响。我们相信，从这项工作中获得的见解将导致发现新的策略来调整分化，以进行原位分化或干细胞治疗。
许多研究人员将间充质干细胞的使用描述为骨再生的一种新疗法。该方法的一个主要障碍是成骨分化的控制。在本文中，作者巧妙地描述了他们的微流控系统，其中载有地塞米松的纳米粒子被生产出来。该系统将允许精确制造纳米粒子，从而提高细胞分化的效率。
