Baričević Ana, Štifanić Mauro, Hamer Bojan, Batel Renato
Ruđer Boskovic Institute, Center for Marine Research, Giordano Paliaga 5, 52210 Rovinj, Croatia.
Juraj Dobrila University, 52100 Pula, Croatia.
Comp Biochem Physiol B Biochem Mol Biol. 2015 Aug;186:51-8. doi: 10.1016/j.cbpb.2015.04.011. Epub 2015 Apr 29.
Roles of p53 family ancestor (p63) in the organisms' response to stressful environmental conditions (mainly pollution) have been studied among molluscs, especially in the genus Mytilus, within the last 15 years. Nevertheless, information about gene structure of this regulatory gene in molluscs is scarce. Here we report the first complete genomic structure of the p53 family orthologue in the mollusc Mediterranean mussel Mytilus galloprovincialis and confirm its similarity to vertebrate p63 gene. Our searches within the available molluscan genomes (Aplysia californica, Lottia gigantea, Crassostrea gigas and Biomphalaria glabrata), found only one p53 family member present in a single copy per haploid genome. Comparative analysis of those orthologues, additionally confirmed the conserved p63 gene structure. Conserved p63 gene structure can be a helpful tool to complement or/and revise gene annotations of any future p63 genomic sequence records in molluscs, but also in other animal phyla. Knowledge of the correct gene structure will enable better prediction of possible protein isoforms and their functions. Our analyses also pointed out possible mis-annotations of the p63 gene in sequenced molluscan genomes and stressed the value of manual inspection (based on alignments of cDNA and protein onto the genome sequence) for a reliable and complete gene annotation.
在过去15年里，人们对软体动物，尤其是贻贝属动物中p53家族祖先基因（p63）在生物体应对应激环境条件（主要是污染）中的作用进行了研究。然而，关于软体动物中这个调控基因的基因结构的信息却很少。在此，我们报告了软体动物地中海贻贝（Mytilus galloprovincialis）中p53家族直系同源基因的首个完整基因组结构，并证实了它与脊椎动物p63基因的相似性。我们在现有的软体动物基因组（加州海兔、加州海蛞蝓、太平洋牡蛎和光滑双脐螺）中进行搜索，发现每个单倍体基因组中仅存在一个单拷贝的p53家族成员。对这些直系同源基因的比较分析进一步证实了p63基因结构的保守性。保守的p63基因结构可以作为一个有用的工具，用于补充或/和修正软体动物以及其他动物门中未来任何p63基因组序列记录的基因注释。了解正确的基因结构将有助于更好地预测可能的蛋白质异构体及其功能。我们的分析还指出了已测序的软体动物基因组中p63基因可能存在的错误注释，并强调了人工检查（基于cDNA和蛋白质与基因组序列的比对）对于可靠和完整的基因注释的价值。
