Center for Evolutionary and Theoretical Immunology, Department of Biology, University of New Mexico, Albuquerque, NM, 87131, USA.
Program in Public Health, College of Health Sciences, University of California, Irvine, CA, 92697, USA.
Commun Biol. 2022 Sep 9;5(1):940. doi: 10.1038/s42003-022-03844-5.
The freshwater snail Biomphalaria glabrata is an important intermediate host of the parasite Schistosoma mansoni that causes human intestinal schistosomiasis. To better understand vector snail biology and help advance innovative snail control strategies, we have developed a new snail model consisting of two homozygous B. glabrata lines (iM line and iBS90) with sharply contrasting schistosome-resistance phenotypes. We produced and compared high-quality genome sequences for iM line and iBS90 which were assembled from 255 (N50 = 22.7 Mb) and 346 (N50 = 19.4 Mb) scaffolds, respectively. Using F2 offspring bred from the two lines and the newly generated iM line genome, we constructed 18 linkage groups (representing the 18 haploid chromosomes) covering 96% of the genome and identified three new QTLs (quantitative trait loci), two involved in snail resistance/susceptibility and one relating to body pigmentation. This study provides excellent genomic resources for unveiling complex vector snail biology, reveals genomic difference between resistant and susceptible lines, and offers novel insights into genetic mechanism of the compatibility between snail and schistosome.
光滑双脐螺是曼氏血吸虫的重要中间宿主，可引起人体肠道血吸虫病。为了更好地了解病媒蜗牛生物学，并帮助推进创新的蜗牛控制策略，我们开发了一种由两个纯合光滑双脐螺系（iM 系和 iBS90 系）组成的新型蜗牛模型，它们具有明显不同的血吸虫抗性表型。我们分别对 iM 系和 iBS90 系进行了高质量基因组序列的组装，其组装分别由 255 个（N50=22.7 Mb）和 346 个（N50=19.4 Mb）支架组成。利用来自这两个系的 F2 后代和新生成的 iM 系基因组，我们构建了 18 个连锁群（代表 18 条单倍体染色体），覆盖了基因组的 96%，并鉴定出了三个新的 QTL（数量性状位点），其中两个与蜗牛的抗性/敏感性有关，一个与身体色素沉着有关。这项研究为揭示复杂的病媒蜗牛生物学提供了极好的基因组资源，揭示了抗性和易感系之间的基因组差异，并为蜗牛与血吸虫之间的相容性的遗传机制提供了新的见解。
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