Li Yansen, Jing Changqing, Chen Yuezhi, Wang Jinshen, Zhou Mingliang, Liu Xin, Sun Dong, Mu Linjun, Li Leping, Guo Xiaobo
Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
Mol Med Rep. 2015 Aug;12(2):2636-42. doi: 10.3892/mmr.2015.3690. Epub 2015 Apr 27.
Tumor necrosis factor α-induced protein 8 (TNFAIP8) has been associated with the tumorigenicity of various types of cancer, however, the expression of TNFAIP8 and its function in gastric cancer remain to be fully elucidated. Therefore, the present study examined the expression and biological function of TNFAIP8 in gastric cancer. The expression levels of TNFAIP8 were determined in 86 gastric cancer tissue samples and adjacent normal tissues using immunohistochemistry, and in four gastric cancer cell lines and GES-1 cells using reverse transcription-quantitative polymerase chain reaction. The expression of TNFAIP8 and its association with the tumor, node, metastasis (TNM) status and lymphatic metastasis of gastric cancer was evaluated. Furthermore, the functions of decreased expression levels of TNFAIP8 were analyzed in human gastric cancer cell lines. The expression of TNFAIP8 was significantly upregulated in the gastric cancer tissues and in the gastric cancer cell lines, and its expression levels were associated with the TNM staging and lymphatic metastasis. Furthermore, decreased expression of TNFAIP8 inhibited the growth, invasion and migration of gastric cancer cells. These data provided an innovative insight suggesting the downregulation of TNFAIP8 as a meaningful approach for treating human gastric cancer and other types of cancer. In addition, the expression levels of TNFAIP8 may be considered as a biomarker of gastric cancer progression.
肿瘤坏死因子α诱导蛋白8（TNFAIP8）与多种癌症的致瘤性有关，然而，TNFAIP8在胃癌中的表达及其功能仍有待充分阐明。因此，本研究检测了TNFAIP8在胃癌中的表达及生物学功能。采用免疫组织化学方法检测86例胃癌组织样本及癌旁正常组织中TNFAIP8的表达水平，采用逆转录-定量聚合酶链反应检测4种胃癌细胞系和GES-1细胞中TNFAIP8的表达水平。评估TNFAIP8的表达及其与胃癌肿瘤、淋巴结、转移（TNM）状态和淋巴转移的关系。此外，还分析了TNFAIP8表达水平降低在人胃癌细胞系中的功能。TNFAIP8在胃癌组织和胃癌细胞系中表达明显上调，其表达水平与TNM分期和淋巴转移有关。此外，TNFAIP8表达降低抑制了胃癌细胞的生长、侵袭和迁移。这些数据提供了一个创新性的见解，表明下调TNFAIP8是治疗人类胃癌和其他类型癌症的一种有意义的方法。此外，TNFAIP8的表达水平可被视为胃癌进展的生物标志物。
