Güler S, Gürkan H, Tozkir H, Turan N, Çelik Y
Department of Neurology, Trakya University Faculty of Medicine, Edirne, Turkey.
Department of Genetics, Trakya University Faculty of Medicine, Edirne, Turkey.
Balkan J Med Genet. 2015 Apr 10;17(2):49-59. doi: 10.2478/bjmg-2014-0074. eCollection 2014 Dec.
We investigated the phenotype-genotype association of the following endothelial nitric oxide synthase (eNOS) gene polymorphisms, rs743506, rs2070744, rs1799983, rs180079, rs3918226, rs207468799 and rs148554851, in patients suffering from migraine living in Edirne, Turkey. A total of 175 individuals, who had been diagnosed with migraine between April 2013 and December 2013, at the Neurology Department, Trakya University Medical Faculty, Edirne, Turkey, and 125 healthy controls were recruited. The above gene polymorphisms were analyzed from genomic DNA in both patient and control groups, using the pyro-sequencing method. The eNOS rs1799983 TT genotype frequency in migraine patients who had a headache duration of longer than 24 hours was statistically significantly higher than in patients who had migraine attacks that lasted under 24 hours (p = 0.047). In terms of the AGGTGGA haplotype, the severity of headache was statistically significant, and was found to be severe in 61.0% (p = 0.0001). Also in terms of the AGGTGGA haplotype, the duration of headache was statistically significant, and was >24 hours in 56.0% of patients (p = 0.008). In our study, there was no significant genotypephenotype relationship between eNOS rs743506, rs2070744, rs1799983, rs180079, rs3918226, rs207468799 and rs148554851 gene polymorphisms and migraine patients with and without aura living in Edirne, Turkey. The AGGTGGA haplotype constitutes a risk in terms of the severity and the duration of headaches in patients with migraine. This risk is significantly higher in patients with migraine with aura than patients with migraine without aura.
我们调查了土耳其埃迪尔内偏头痛患者中以下内皮型一氧化氮合酶（eNOS）基因多态性，即rs743506、rs2070744、rs1799983、rs180079、rs3918226、rs207468799和rs148554851的表型-基因型关联。共招募了175名个体，他们于2013年4月至2013年12月期间在土耳其埃迪尔内特拉凯亚大学医学院神经科被诊断为偏头痛，以及125名健康对照者。使用焦磷酸测序法对患者组和对照组的基因组DNA进行上述基因多态性分析。头痛持续时间超过24小时的偏头痛患者中eNOS rs1799983 TT基因型频率在统计学上显著高于偏头痛发作持续时间在24小时以内的患者（p = 0.047）。就AGGTGGA单倍型而言，头痛严重程度具有统计学意义，61.0%的患者为重度（p = 0.0001）。同样就AGGTGGA单倍型而言，头痛持续时间具有统计学意义，56.0%的患者头痛持续时间>24小时（p = 0.008）。在我们的研究中，土耳其埃迪尔内有先兆和无先兆偏头痛患者中，eNOS rs743506、rs2070744、rs1799983、rs180079、rs3918226、rs207468799和rs148554851基因多态性与偏头痛患者之间不存在显著的基因型-表型关系。AGGTGGA单倍型在偏头痛患者头痛的严重程度和持续时间方面构成风险。这种风险在有先兆偏头痛患者中显著高于无先兆偏头痛患者。
