Safaei Tina Saberi, Mohamadi Reza M, Sargent Edward H, Kelley Shana O
†Department of Electrical and Computer Engineering, Faculty of Applied Science and Engineering, University of Toronto, 10 King's College Road, Toronto, Ontario M5S 3G4, Canada.
‡Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, 144 College Street, Toronto, Ontario M5S 3M2, Canada.
ACS Appl Mater Interfaces. 2015 Jul 8;7(26):14165-9. doi: 10.1021/acsami.5b02404. Epub 2015 May 13.
Circulating tumor cells (CTCs) are cancer cells disseminated from a tumor into the bloodstream. Their presence in patient blood samples has been associated with metastatic disease. Here, we report a simple system that enables the isolation and detection of these rare cancer cells. By developing a sensitive electrochemical ELISA method integrated within a microfluidic cell capture system, were we able to reliably detect very low levels of cancer cells in whole blood. Our results indicate that the new system provides the clinically relevant specificity and sensitivity needed for a convenient, point-of-need assay for cancer cell counting.
循环肿瘤细胞（CTCs）是从肿瘤扩散到血液中的癌细胞。它们在患者血液样本中的存在与转移性疾病有关。在此，我们报告了一种简单的系统，可用于分离和检测这些罕见的癌细胞。通过开发一种集成在微流控细胞捕获系统中的灵敏电化学ELISA方法，我们能够可靠地检测全血中极低水平的癌细胞。我们的结果表明，新系统提供了方便的即时检测癌细胞计数所需的临床相关特异性和灵敏度。
