Lu Qiaomei, Zhang Wenmin, Gao Jia, Lu Minghua, Zhang Lan, Li Jianrong
Analytical and Testing Center, Fuzhou University, Fuzhou, Fujian, 350002, China; Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350002, Fujian, China.
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350002, Fujian, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jun 15;992:8-13. doi: 10.1016/j.jchromb.2015.04.014. Epub 2015 Apr 18.
Fruit development is influenced greatly by endogenous hormones including salicylic acid (SA) and abscisic acid (ABA). Mass spectrometry with high sensitivity has become a routine technology to analyze hormones. However, pretreatment of plant samples remains a difficult problem. Thus, dispersive liquid-liquid microextraction (DLLME) was used to concentrate trace plant hormones before liquid chromatography-ion trap mass spectrometry (LC-ITMS) analysis. Standard curves were linear within the ranges of 0.5-50, 0.2-20ng/mL for SA and ABA, respectively. The correlation coefficients were greater than 0.9995 with recoveries above 87.5%. The limits of detection were 0.2ng/mL for SA and 0.1ng/mL for ABA in spiked water solution, respectively (injection 20μL). The successful analysis of SA and ABA in fruit samples indicated our DLLME-LC-ITMS approach was efficient, allowing reliable quantification of both two compounds from very small amounts of plant material. Moreover, this research revealed the relationship between SA and ABA content and development of peach fruit at different growth stages.
果实发育受到包括水杨酸（SA）和脱落酸（ABA）在内的内源激素的极大影响。高灵敏度质谱已成为分析激素的常规技术。然而，植物样品的预处理仍然是一个难题。因此，在液相色谱 - 离子阱质谱（LC - ITMS）分析之前，采用分散液液微萃取（DLLME）来浓缩痕量植物激素。SA和ABA的标准曲线分别在0.5 - 50、0.2 - 20 ng/mL范围内呈线性。相关系数大于0.9995，回收率高于87.5%。在加标水溶液中，SA的检测限为0.2 ng/mL，ABA的检测限为0.1 ng/mL（进样20μL）。果实样品中SA和ABA的成功分析表明我们的DLLME - LC - ITMS方法是有效的，能够从极少量的植物材料中可靠地定量这两种化合物。此外，本研究揭示了不同生长阶段桃果实中SA和ABA含量与果实发育之间的关系。
