Owen Stacey J, Abrams Suzanne R
National Research Council of Canada-Plant Biotechnology Institute, Saskatoon, Saskatchewan, Canada.
Methods Mol Biol. 2009;495:39-51. doi: 10.1007/978-1-59745-477-3_4.
Abscisic acid (ABA) plays a number of key roles in the growth, development, and stress response of plants. For example, it is vital to a plant's response to drought stress, and is the signalling molecule responsible for closure of the stomata in order to promote water conservation. The hormone is rapidly turned over in plant tissue, mainly by oxidation or conjugation. Accurate and sensitive quantification of ABA and its metabolites has made a significant contribution to the knowledge of the role of this hormone, and also of its relationship to the induction of numerous ABA-induced genes in plants. High-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has become an essential technique for the analysis and quantification of these compounds.
脱落酸（ABA）在植物的生长、发育和应激反应中发挥着多种关键作用。例如，它对于植物应对干旱胁迫至关重要，并且是负责气孔关闭以促进水分保持的信号分子。该激素在植物组织中主要通过氧化或结合作用迅速周转。对ABA及其代谢物进行准确而灵敏的定量分析，为了解这种激素的作用以及它与植物中众多ABA诱导基因的诱导之间的关系做出了重大贡献。高效液相色谱与串联质谱联用（LC-MS/MS）已成为分析和定量这些化合物的一项重要技术。
