Ma Jun, Du Guangyue, Li Xihuan, Zhang Caiying, Guo Jinkao
School of Plant Biology, The University of Western Australia, Perth, WA, 6009, Australia.
CSIRO Agriculture Flagship, 306 Carmody Road, Brisbane, QLD, 4067, Australia.
Mol Genet Genomics. 2015 Oct;290(5):1955-62. doi: 10.1007/s00438-015-1053-3. Epub 2015 May 5.
Malondialdehyde (MDA) is a naturally occurring product of lipid peroxidation and the level of MDA in plant is often used as a parameter to evaluate the damage to plants' cells due to stress. Plant with lower amounts of MDA under drought conditions is generally considered as more tolerant to drought. In this study, a population of recombinant inbred lines was used to map the quantitative trait locus (QTLs) that controlled MDA content under well-watered condition (WW) and water deficit (WD) condition. A major QTL, designated as Qheb.mda-3B, was detected on the long arm of chromosome 3B. Based on interval mapping analysis, Qheb.mda-3B explained 31.5 and 39.0 % of the phenotypic variance under WW and WD conditions, respectively. Qheb.mda-3B was located in the same interval as a previously identified QTL (Qcrs.cpi-3B) that controlled resistance to Fusarium crown rot (FCR), a fungal disease caused by Fusarium species. Three pairs of near-isogenic lines (NILs) previously developed for Qcrs.cpi-3B were found to show significant differences in MDA content under WD condition. These results suggested that same set of genes is likely to be involved in drought tolerance and FCR resistance in wheat.
丙二醛（MDA）是脂质过氧化的天然产物，植物中MDA的含量常被用作评估胁迫对植物细胞造成损伤的一个参数。在干旱条件下，MDA含量较低的植物通常被认为对干旱的耐受性更强。在本研究中，利用重组自交系群体对在充分供水条件（WW）和水分亏缺（WD）条件下控制MDA含量的数量性状位点（QTL）进行定位。在3B染色体长臂上检测到一个主效QTL，命名为Qheb.mda - 3B。基于区间作图分析，Qheb.mda - 3B在WW和WD条件下分别解释了31.5%和39.0%的表型变异。Qheb.mda - 3B位于与先前鉴定的控制对镰刀菌冠腐病（FCR，一种由镰刀菌属引起的真菌病害）抗性的QTL（Qcrs.cpi - 3B）相同的区间内。发现先前为Qcrs.cpi - 3B构建的三对近等基因系（NILs）在WD条件下MDA含量存在显著差异。这些结果表明，同一组基因可能参与了小麦的耐旱性和对FCR的抗性。
