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基于适配体门控金纳米笼的生物响应性控释生物测定及其在活细胞中的应用。

A bioresponsive controlled-release bioassay based on aptamer-gated Au nanocages and its application in living cells.

作者信息

Wang Wei, Chen Chen, Li Xiaoxiao, Wang Shiying, Luo Xiliang

机构信息

Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.

出版信息

Chem Commun (Camb). 2015 Jun 4;51(44):9109-12. doi: 10.1039/c5cc02452h.

DOI:10.1039/c5cc02452h
PMID:25939588
Abstract

A novel controlled-release bioassay for highly sensitive and selective detection of intracellular ATP was developed based on positive charge-modified Au nanocages capped with an aptamer molecular gate, and it was used successfully for fluorescence microscopy imaging of ATP in living cells.

摘要

基于用适配体分子门封端的正电荷修饰金纳米笼,开发了一种用于高灵敏度和选择性检测细胞内ATP的新型控释生物测定法,并成功用于活细胞中ATP的荧光显微镜成像。

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