Godbert Sonya, Miro Fernando, Shreeves Chris, Gnoth Christian, Johnson Sarah
SPD Development Company Ltd, Priory Business Park, Stannard Way, Bedford, MK44 3UP, UK.
green-ivf, Grevenbroich Endocrinology and IVF-Center, Rheydter Str. 143, 41515, Grevenbroich, Germany.
Arch Gynecol Obstet. 2015 Nov;292(5):1153-61. doi: 10.1007/s00404-015-3732-z. Epub 2015 May 5.
To determine whether an optimal method exists for the detection of the luteinising hormone (LH) surge onset in research datasets of urinary hormonal profiles of menstrual cycles.
The scientific literature was searched to compare published methodologies for detection of the LH surge onset in urine. Their performance was tested using complete hormonal profiles from 254 ovulatory cycles from 227 women attempting pregnancy (normal regular menstrual cycles; no known infertility).
Three major methodologies to determine the onset of the LH surge in urine were identified. The key difference between these methods is how the cycle days that contribute to LH baseline assessment are determined: using fixed days (method #1), based on peak LH day (method #2), based on a provisional estimate of the LH surge (method #3). Method #1 requires no prior cycle information, whereas methods #2 and #3 need to consider complete cycle data. The most reliable method for calculation of baseline LH was using 2 days before the estimated surge day, plus the previous 4/5 days.
Different methods for identification of the urinary LH surge can provide very different determinations of LH surge day, thus care must be taken when comparing between studies that apply different methodologies. The optimal method for determining the onset of the LH surge in urine requires retrospective estimation of day of LH surge to identify the most appropriate part of the cycle to consider as the baseline. This method can be adopted for application in population studies.
确定在月经周期尿激素谱研究数据集中，是否存在检测促黄体生成素（LH）峰起始的最佳方法。
检索科学文献，以比较已发表的检测尿中LH峰起始的方法。使用来自227名尝试怀孕女性（正常规律月经周期；无已知不孕症）的254个排卵周期的完整激素谱对这些方法的性能进行测试。
确定了三种主要的检测尿中LH峰起始的方法。这些方法之间的关键区别在于如何确定用于LH基线评估的周期天数：使用固定天数（方法#1）、基于LH峰值日（方法#2）、基于LH峰的初步估计（方法#3）。方法#1不需要先前的周期信息，而方法#2和#3需要考虑完整的周期数据。计算基线LH最可靠的方法是使用估计的LH峰日之前的2天加上前4/5天。
不同的识别尿LH峰的方法对LH峰日的判定可能差异很大，因此在比较应用不同方法的研究时必须谨慎。确定尿中LH峰起始的最佳方法需要对LH峰日进行回顾性估计，以确定周期中最适合作为基线的部分。该方法可用于人群研究。
