Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz (Germany).
Department of Hematology, Medical Oncology, and Pneumology, University Medical Center Mainz, Langenbeckstr. 1, 55131 Mainz (Germany).
Angew Chem Int Ed Engl. 2015 Jun 15;54(25):7436-40. doi: 10.1002/anie.201502398. Epub 2015 May 4.
Whenever nanoparticles encounter biological fluids like blood, proteins adsorb on their surface and form a so-called protein corona. Although its importance is widely accepted, information on the influence of surface functionalization of nanocarriers on the protein corona is still sparse, especially concerning how the functionalization of PEGylated nanocarriers with targeting agents will affect protein corona formation and how the protein corona may in turn influence the targeting effect. Herein, hydroxyethyl starch nanocarriers (HES-NCs) were prepared, PEGylated, and modified on the outer PEG layer with mannose to target dendritic cells (DCs). Their interaction with human plasma was then studied. Low overall protein adsorption with a distinct protein pattern and high specific affinity for DC binding were observed, thus indicating an efficient combination of "stealth" and targeting behavior.
当纳米颗粒遇到像血液这样的生物流体时，蛋白质会吸附在其表面并形成所谓的蛋白质冠。尽管其重要性已被广泛接受，但关于纳米载体表面功能化对蛋白质冠形成的影响的信息仍然很少，特别是关于带有靶向剂的聚乙二醇化纳米载体的功能化将如何影响蛋白质冠形成，以及蛋白质冠又将如何反过来影响靶向效果。在此，制备了羟乙基淀粉纳米载体（HES-NC），并在其外层聚乙二醇层上用甘露糖进行了聚乙二醇化修饰，以靶向树突状细胞（DC）。然后研究了它们与人血浆的相互作用。观察到总蛋白吸附量低，具有明显的蛋白质模式，并且对 DC 结合具有高特异性亲和力，从而表明“隐身”和靶向行为的有效结合。
