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用于早期鉴定血培养瓶中生长细菌的基质辅助激光解吸电离飞行时间质谱分析

MALDI-TOF mass spectrometry for early identification of bacteria grown in blood culture bottles.

作者信息

Zabbe Jean-Benoît, Zanardo Laura, Mégraud Francis, Bessède Emilie

机构信息

Laboratory of Bacteriology, University Hospital of Bordeaux, France.

出版信息

J Microbiol Methods. 2015 Aug;115:45-6. doi: 10.1016/j.mimet.2015.04.009. Epub 2015 May 2.

DOI:10.1016/j.mimet.2015.04.009
PMID:25940929
Abstract

This note reports an interesting way to rapidly identify bacteria grown from blood culture bottles. Chocolate agar plates were inoculated with 1 drop of the positive blood bottle medium. After a 3-hour incubation, the growth veil was submitted to MALDI-TOF mass spectrometry: 77% of the bacteria present have been correctly identified.

摘要

本笔记报告了一种快速鉴定从血培养瓶中培养出的细菌的有趣方法。用一滴阳性血瓶培养基接种巧克力琼脂平板。经过3小时培养后，将生长菌膜进行基质辅助激光解吸电离飞行时间质谱分析：77%的存在细菌得到了正确鉴定。

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