通过基质辅助激光解吸电离飞行时间质谱法（Vitek® MS）从阳性血培养沉淀物中快速鉴定细菌的四种方案评估。
Assessment of four protocols for rapid bacterial identification from positive blood culture pellets by matrix-assisted laser desorption ionization-time of flight mass spectrometry (Vitek® MS).
作者信息
Thomin Jean, Aubin Guillaume Ghislain, Foubert Fabrice, Corvec Stéphane
机构信息
Service de Bactériologie-Hygiène hospitalière, Nantes University Hospital, France.
Service de Bactériologie-Hygiène hospitalière, Nantes University Hospital, France; EA3826, Thérapeutiques cliniques et expérimentales des infections - Nantes Medicine school, France.
出版信息
J Microbiol Methods. 2015 Aug;115:54-6. doi: 10.1016/j.mimet.2015.05.010. Epub 2015 May 9.
In this study, we developed and compared four protocols to prepare a bacterial pellet from 944 positive blood cultures for direct MALDI-TOF mass spectrometry Vitek® MS analysis. Protocol 4, tested on 200 monomicrobial samples, allowed 83% of bacterial identification. This easy, fast, cheap and accurate method is promising in daily practice, especially to limit broad range antibiotic treatment.
在本研究中，我们开发并比较了四种从944份阳性血培养物中制备细菌沉淀以用于直接基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）Vitek® MS分析的方案。在200份单微生物样本上测试的方案4实现了83%的细菌鉴定率。这种简便、快速、廉价且准确的方法在日常实践中很有前景，特别是在限制广谱抗生素治疗方面。
Zotero 插件