Zhang Xueping, Liu Dong, Li Libo, You Tianyan
1] State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China [2] University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
Sci Rep. 2015 May 6;5:9885. doi: 10.1038/srep09885.
We have proposed a novel free-standing nitrogen-doped carbon nanospheres@carbon nanofibers (NCNSs@CNFs) composite film with high processability for the investigation of the direct electron transfer (DET) of glucose oxidase (GOx) and the DET-based glucose biosensing. The composites were simply prepared by controlled thermal treatment of electrospun polypyrrole nanospheres doped polyacrylonitrile nanofibers (PPyNSs@PAN NFs). Without any pretreatment, the as-prepared material can directly serve as a platform for GOx immobilization. The cyclic voltammetry of immobilized GOx showed a pair of well-defined redox peaks in O2-free solution, indicating the DET of GOx. With the addition of glucose, the anodic peak current increased, while the cathodic peak current decreased, which demonstrated the DET-based bioelectrocatalysis. The detection of glucose based on the DET of GOx was achieved, which displayed high sensitivity, stability and selectivity, with a low detection limit of 2 μM and wide linear range of 12-1000 μM. These results demonstrate that the as-obtained NCNSs@CNFs can serve as an ideal platform for the construction of the third-generation glucose biosensor.
我们提出了一种具有高加工性能的新型自支撑氮掺杂碳纳米球@碳纳米纤维（NCNSs@CNFs）复合膜，用于研究葡萄糖氧化酶（GOx）的直接电子转移（DET）以及基于DET的葡萄糖生物传感。通过对静电纺丝的聚吡咯纳米球掺杂聚丙烯腈纳米纤维（PPyNSs@PAN NFs）进行可控热处理，可简单制备出该复合材料。未经任何预处理，所制备的材料即可直接用作固定GOx的平台。固定化GOx的循环伏安法在无氧溶液中显示出一对明确的氧化还原峰，表明存在GOx的直接电子转移。加入葡萄糖后，阳极峰电流增加，而阴极峰电流降低，这证明了基于DET的生物电催化作用。实现了基于GOx直接电子转移的葡萄糖检测，该检测具有高灵敏度、稳定性和选择性，检测限低至2 μM，线性范围宽达12 - 1000 μM。这些结果表明，所获得的NCNSs@CNFs可作为构建第三代葡萄糖生物传感器的理想平台。
