从零售绞肉中分离出的葡萄球菌的物种多样性以及表型和基因型抗生素耐药模式
Species Diversity and Pheno- and Genotypic Antibiotic Resistance Patterns of Staphylococci Isolated from Retail Ground Meats.
作者信息
Guran Husnu Sahan, Kahya Serpil
机构信息
Dept. of Food Hygiene and Technology, Faculty of Veterinary Medicine, Dicle Univ, 21280, Diyarbakir, Turkey.
Dept. of Microbiology, Faculty of Veterinary Medicine, Uludag Univ, 16120, Bursa, Turkey.
出版信息
J Food Sci. 2015 Jun;80(6):M1291-8. doi: 10.1111/1750-3841.12893. Epub 2015 May 5.
The presence and species diversity of staphylococci in 250 ground beef and lamb meat samples obtained from Diyarbakir, Turkey were investigated. The presence of the 16S rRNA gene, mecA, nuc, pvl, and femA was analyzed by multiplex PCR. Pheno- and genotypic antibiotic resistance profiles of 208 staphylococci isolates were established. Of the ground beef and ground lamb samples, 86.4% and 62.4% were positive for staphylococci, respectively. Staphylococcus aureus, S. saprophyticus, S. hominis, S. lentus, S. pasteuri, S. warneri, S. intermedius, and S. vitulinus made up 40.8%, 28.8%, 11%, 3.8%, 3.8%, 2.4%, 2.4%, and 2.4% of isolates, respectively. Of the 85 S. aureus isolates, 40%, 47%, and 5.8% carried femA, mecA, and pvl, respectively, whereas the corresponding rates for the 118 coagulase-negative staphylococci (CoNS) were 0%, 10.1%, and 0%, respectively. We determined from the 208 isolates, the highest antibiotic resistances were to tetracycline and oxytetracycline (85.5%), followed by penicillin (51.4%), novobiocin (45.6%), ampicillin (39.9%), and doxycycline (31.7%), using the Clinical and Laboratory Standards Inst. (CLSI) method. All isolates were sensitive to gentamycin, ofloxacin, and tobramycin, but 2.3% of the S. aureus isolates had resistance to vancomycin. The staphylococci isolates carried tet(K), blaZ, tet(L), tet(W), cat, tet(S), tet(M), ermB, ermA, and ermC antibiotic resistance genes at rates of 59%, 51.7%, 36.9%, 31.8%, 27.2%, 27.2%, 24.4%, 18.1%, 7.9%, and 3.9%, respectively.
对从土耳其迪亚巴克尔采集的250份牛肉末和羊肉末样本中的葡萄球菌存在情况及物种多样性进行了调查。通过多重聚合酶链反应分析了16S rRNA基因、mecA、nuc、pvl和femA的存在情况。确定了208株葡萄球菌分离株的表型和基因型抗生素耐药谱。在牛肉末和羊肉末样本中，葡萄球菌阳性率分别为86.4%和62.4%。金黄色葡萄球菌、腐生葡萄球菌、人葡萄球菌、缓慢葡萄球菌、巴斯德葡萄球菌、沃氏葡萄球菌、中间葡萄球菌和小牛葡萄球菌分别占分离株的40.8%、28.8%、11%、3.8%、3.8%、2.4%、2.4%和2.4%。在85株金黄色葡萄球菌分离株中，分别有40%、47%和5.8%携带femA、mecA和pvl，而118株凝固酶阴性葡萄球菌（CoNS）的相应比例分别为0%、10.1%和0%。根据临床和实验室标准协会（CLSI）的方法，我们从208株分离株中确定，最高的抗生素耐药率是对四环素和土霉素（85.5%），其次是青霉素（51.4%）、新生霉素（45.6%）、氨苄青霉素（39.9%）和强力霉素（31.7%）。所有分离株对庆大霉素、氧氟沙星和妥布霉素敏感，但2.3%的金黄色葡萄球菌分离株对万古霉素耐药。葡萄球菌分离株携带tet(K)、blaZ、tet(L)、tet(W)、cat、tet(S)、tet(M)、ermB、ermA和ermC抗生素耐药基因的比例分别为59%、51.7%、36.9%、31.8%、27.2%、27.2%、24.4%、18.1%、7.9%和3.9%。
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