Shivani Y, Subhash Y, Dave Bharti P, Sasikala Ch, Ramana Ch V
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J.N.T. University Hyderabad, Kukatpally, Hyderabad 500 085, India.
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500 046, India.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2531-2536. doi: 10.1099/ijs.0.000296. Epub 2015 May 5.
Two bacterial strains (JC247T and JC248) were isolated from soil samples collected from Rann of Kutch, Gujarat, India. Colonies of both strains were creamy white. Cells were Gram-stain-positive, rods-to-curved rods (crescent-shaped), and produced centrally located oval-shaped endospores. Major (>5 %) fatty acids of both strains were iso-C16 : 0, iso-C14 : 0, iso-C15 : 0, C16 : 1ω11c and C16 : 0, with minor ( < 5 but >1 %) amounts of anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 1 H, iso-C17 : 0, iso-C18 : 0, C14 : 0, C17 : 0, C18 : 0, C18 : 1ω9c, iso-C17 : 1ω10c and anteiso-C17 : 0B/isoI. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids of both strains. Cell-wall amino acids were l-alanine, d-alanine, d-glutamic acid and meso-diaminopimelic acid. The genomic DNA G+C content of strains JC247T and JC248 was 48.2 and 48.1 mol%, respectively. Both strains were closely related with mean DNA-DNA hybridization >90 %. 16S rRNA gene sequence analysis of both strains indicated that they are members of the genus Bacillus within the family Bacillaceae of the phylum Firmicutes. Both strains had a 16S rRNA gene sequence similarity of 96.93 % with Bacillus firmus NCIMB 9366T and < 96.92 % with other members of the genus Bacillus. Sequence similarity between strain JC247T and JC248 was 100 %. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strains JC247T and JC248 as representatives of a novel species of the genus Bacillus, for which the name Bacilluscrescens sp. nov. is proposed. The type strain is JC247T ( = KCTC 33627T = LMG 28608T).
从印度古吉拉特邦库奇兰恩采集的土壤样本中分离出两株细菌菌株（JC247T和JC248）。两株菌株的菌落均为乳脂白色。细胞革兰氏染色呈阳性，为杆状至弯曲杆状（新月形），并产生位于中央的椭圆形内生孢子。两株菌株的主要（>5%）脂肪酸为异-C16 : 0、异-C14 : 0、异-C15 : 0、C16 : 1ω11c和C16 : 0，少量（<5%但>1%）的anteiso-C15 : 0、anteiso-C17 : 0、异-C16 : 1 H、异-C17 : 0、异-C18 : 0、C14 : 0、C17 : 0、C18 : 0、C18 : 1ω9c、异-C17 : 1ω10c和anteiso-C17 : 0B/isoI。二磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油是两株菌株的主要极性脂质。细胞壁氨基酸为L-丙氨酸、D-丙氨酸、D-谷氨酸和内消旋二氨基庚二酸。菌株JC247T和JC248的基因组DNA G+C含量分别为48.2和48.1 mol%。两株菌株密切相关，平均DNA-DNA杂交率>90%。对两株菌株的16S rRNA基因序列分析表明，它们是厚壁菌门芽孢杆菌科芽孢杆菌属的成员。两株菌株与坚强芽孢杆菌NCIMB 9366T的16S rRNA基因序列相似性为96.93%，与芽孢杆菌属其他成员的相似性<96.92%。菌株JC247T和JC248之间的序列相似性为100%。与先前描述的分类单元在形态、生理和基因型上的明显差异支持将菌株JC
