Azmatunnisa Begum M, Varshini V, Rahul K, Chandana A, Sasikala Ch, Ramana Ch V
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University Hyderabad, Kukatpally, Hyderabad 500 085, India.
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500 046, India.
Int J Syst Evol Microbiol. 2016 Nov;66(11):4772-4778. doi: 10.1099/ijsem.0.001428. Epub 2016 Aug 16.
A novel Gram-stain-positive, aerobic, endospore-forming, non-motile, rod-shaped bacterium (strain JC229T) was isolated from a water sample collected from waterlogged alkaline soil. Strain JC229T was oxidase- and catalase-positive. Based on 16S rRNA gene sequence analysis, strain JC229T was identified as belonging to the genus Alteribacillus of the phylum Firmicutes and was found to be most closely related to Alteribacillus bidgolensis P4BT (97.9 % similarity), Bacillus iranensis X5BT (97.2 %) and Alteribacillus persepolensis HS136T (96.6 %), and more distantly related to other members of the genus Bacillus (<95.2 %). Strain JC229T was further identified to be distinctly related to the type strains of A. bidgolensis and B. iranensis (<26 % based on DNA-DNA hybridization and ΔTm of >5 °C). Strain JC229T grew optimally at pH 8 (range 5-11), at 35-40 °C (range 20-50 °C) and at a salinity of 3-5 % (range 0.5-24 %). The DNA G+C content was 40.2 mol%. Major cellular fatty acids of strain JC229T were anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant quinone system was menaquinone 7. Polar lipids of strain JC229T included diphosphatidylglycerol, phosphatidylglycerol and two unidentified lipids. On the basis of morphological, physiological, genetic, phylogenetic and chemotaxonomic analyses, strain JC229T should be assigned to a novel species of the genus Alteribacillus, for which the name Alteribacillus alkaliphilus sp. nov. is proposed. The type strain is JC229T (=LMG 28999T=KCTC 33726T). It is also suggested to transfer B. iranensis to the genus Alteribacillus as Alteribacillus iranensis comb. nov.
从涝渍碱性土壤采集的水样中分离出一株新型革兰氏阳性、需氧、产芽孢、不运动的杆状细菌（菌株JC229T）。菌株JC229T氧化酶和过氧化氢酶呈阳性。基于16S rRNA基因序列分析，菌株JC229T被鉴定为属于厚壁菌门的Alteribacillus属，发现与Bidgolensis Alteribacillus P4BT（相似度97.9%）、伊朗芽孢杆菌X5BT（97.2%）和Persepolensis Alteribacillus HS136T（96.6%）关系最为密切，与芽孢杆菌属的其他成员关系较远（<95.2%）。菌株JC229T经进一步鉴定与Bidgolensis Alteribacillus和伊朗芽孢杆菌的模式菌株明显不同（基于DNA-DNA杂交<26%，ΔTm>5°C）。菌株JC229T在pH 8（范围5 - 11）、35 - 40°C（范围20 - 50°C）和盐度3 - 5%（范围0.5 - 24%）条件下生长最佳。DNA G+C含量为40.2 mol%。菌株JC229T的主要细胞脂肪酸为anteiso-C15 : 0、iso-C15 : 0、anteiso-C17 : 0和iso-C16 : 0。肽聚糖含有内消旋二氨基庚二酸作为诊断性二氨基酸。主要醌系统为甲基萘醌7。菌株JC229T的极性脂质包括双磷脂酰甘油、磷脂酰甘油和两种未鉴定的脂质。基于形态、生理、遗传、系统发育和化学分类分析，菌株JC229T应归为Alteribacillus属的一个新物种，为此提出名为嗜碱Alteribacillus的新种。模式菌株为JC229T（=LMG 28999T=KCTC 33726T）。还建议将伊朗芽孢杆菌转移至Alteribacillus属，命名为Alteribacillus iranensis comb. nov.
