Cai Haiyuan, Wang Yanan, Xu Huacheng, Yan Zaisheng, Jia Bin, Majid Maszenan Abdul, Jiang Helong
State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, PR China.
Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences, Zhengzhou, PR China.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2537-2541. doi: 10.1099/ijs.0.000299. Epub 2015 May 5.
A Gram-stain-negative, slightly curved rod-shaped, non-spore-forming diazotrophic bacterium, designated strain TH16T, was isolated from cyanobacterial aggregates taken from eutrophic Lake Taihu, Jiangsu Province, China. The pH range for growth was 5-9 (optimum at pH 7.0), salinity range was 0-2% (w/v) NaCl (optimum 0%) and temperature range was 20-37 °C (optimum 30 °C) in nutrient broth. Phylogenetic analysis indicated that strain TH16T clusters near and is closely related to the genus Niveispirillum within the family Rhodospirillaceae of the class Alphaproteobacteria. Within the genus Niveispirillum, strain TH16T was related most closely to Niveispirillum irakense KBC1T (98.1% 16S rRNA gene sequence similarity) and Niveispirillum fermenti CC-LY736T (97.0 %). The DNA G+C content of strain TH16T was 64 mol%. DNA-DNA relatedness between strain TH16T and the type strains of N. irakense and N. fermenti was 39.6 and 30.1%, respectively. The major respiratory quinone was ubiquinone Q-10.The major fatty acids (>10%) were C18 : 1ω6c/C18 : 1ω7c, C18 : 1 2-OH and C16 : 0 3-OH. Genes in the puf operon, encoding proteins of the photosynthetic reaction centre and core light-harvesting complexes, were also present. Based on morphological, chemotaxonomic and phylogenetic data, strain TH16T represents a novel species within the genus Niveispirillum, for which the name Niveispirillum cyanobacteriorum sp. nov. is proposed. The type strain is TH16T ( = CGMCC 1.12958T = LMG 28334T).
从中国江苏省富营养化的太湖采集的蓝藻聚集体中分离出一株革兰氏染色阴性、略弯曲的杆状、不形成芽孢的固氮细菌，命名为TH16T菌株。在营养肉汤中，其生长的pH范围为5-9（最适pH为7.0），盐度范围为0-2%（w/v）NaCl（最适0%），温度范围为20-37°C（最适30°C）。系统发育分析表明，TH16T菌株聚集在α-变形菌纲红螺菌科的Niveispirillum属附近，且与该属密切相关。在Niveispirillum属内，TH16T菌株与伊拉克Niveispirillum irakense KBC1T菌株（16S rRNA基因序列相似性为98.1%）和发酵Niveispirillum fermenti CC-LY736T菌株（97.0%）关系最为密切。TH16T菌株的DNA G+C含量为64 mol%。TH16T菌株与伊拉克Niveispirillum irakense和发酵Niveispirillum fermenti模式菌株之间的DNA-DNA相关性分别为39.6%和30.1%。主要呼吸醌为泛醌Q-10。主要脂肪酸（>10%）为C18 : 1ω6c/C18 : 1ω7c、C18 : 1 2-OH和C16 : 0 3-OH。编码光合反应中心和核心光捕获复合体蛋白质的puf操纵子中的基因也存在。基于形态、化学分类和系统发育数据，TH16T菌株代表Niveispirillum属内的一个新物种，为此提出新名称蓝藻Niveispirillum cyanobacteriorum sp. nov.。模式菌株为TH16T（ = CGMCC 1.12958T = LMG 28334T）。
