Cai Haiyuan, Zeng Yonghui, Wang Yanan, Cui Henglin, Jiang Helong
State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, PR China.
Aarhus Institute of Advanced Studies and Department of Environmental Science, Aarhus University, Denmark, Denmark.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1279-1284. doi: 10.1099/ijsem.0.002664. Epub 2018 Feb 27.
A Gram-reaction-negative, aerobic, non-flagellated, non-gliding, rod-shaped and yellow-pigmented bacterium, designated strain TH021, was isolated from cyanobacterial aggregates in a eutrophic lake, Taihu Lake, China. Optimal growth occurred at pH 7.0 (range: 5.0-10.0), 28 °C (range, 4-32 °C) and 0 % (w/v) NaCl (range, 0-1.0 %) in Reasoner's 2A broth. No growth was observed at 37 °C. The cells were found to be positive for oxidase and catalase activities. The major respiratory quinone was menaquinone-6. The major fatty acids (>10 %) were identified as iso-C15 : 0 and C16 : 1ω7c/C16 : 1ω6c. The major polar lipid was phosphatidylethanolamine. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was affiliated with the genus Flavobacterium, with the highest sequence similarity found to Flavobacterium hauense BX12 (94.92 %), followed by Flavobacterium suzhouense XIN-1 (94.85 %), Flavobacterium arcticum SM1502 (94.79 %) and Flavobacterium beibuense F44-8 (94.30 %). The genomic G+C content of strain TH021 was 41.9 mol% based on total genome calculations. Average nucleotide identities and digital DNA-DNA hybridizations values for complete genomes ranged from 69.4 to 72.8 and 18.0 to 23.8 % between strain TH021 and strains within the genus Flavobacterium. The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis suggested that strain TH021 represents a novel species within the genus Flavobacterium, for which the name Flavobacterium cyanobacteriorum sp. nov. is proposed. The type strain is TH021 (=LMG 29720=CGMCC 1.16325).
从中国富营养化湖泊太湖的蓝藻聚集体中分离出一株革兰氏阴性、需氧、无鞭毛、不滑动、杆状且产黄色色素的细菌，命名为TH021菌株。在瑞氏2A肉汤中，最适生长条件为pH 7.0（范围：5.0 - 10.0）、28 °C（范围：4 - 32 °C）和0 %（w/v）NaCl（范围：0 - 1.0 %）。在37 °C未观察到生长。发现细胞氧化酶和过氧化氢酶活性呈阳性。主要呼吸醌为甲基萘醌-6。主要脂肪酸（>10 %）鉴定为异-C15 : 0和C16 : 1ω7c/C16 : 1ω6c。主要极性脂为磷脂酰乙醇胺。基于16S rRNA基因序列的系统发育分析表明，该分离株属于黄杆菌属，与黄杆菌属的哈氏黄杆菌BX12（94.92 %）序列相似性最高，其次是苏州黄杆菌XIN-1（94.85 %）、北极黄杆菌SM1502（94.79 %）和北部湾黄杆菌F44-8（94.30 %）。基于全基因组计算，TH021菌株的基因组G+C含量为41.9 mol%。TH021菌株与黄杆菌属内其他菌株的全基因组平均核苷酸同源性和数字DNA - DNA杂交值分别为69.4%至72.8%和18.0%至23.8%。表型、化学分类和系统发育特性以及基因组分析表明，TH021菌株代表黄杆菌属内的一个新物种，为此提议将其命名为蓝藻黄杆菌（Flavobacterium cyanobacteriorum sp. nov.）。模式菌株为TH021（=LMG 29720 = CGMCC 1.16325）。
