Cai Haiyuan, Cui Henglin, Zeng Yonghui, An Mingli, Jiang Helong
State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, PR China.
School of Food & Biological Engineering, Jiangsu University, Zhenjiang, PR China.
Int J Syst Evol Microbiol. 2018 Mar;68(3):730-735. doi: 10.1099/ijsem.0.002571. Epub 2018 Jan 15.
A bacterial strain, designated TH057, was isolated from cyanobacterial aggregates in a eutrophic lake in China. Cells were observed to be slightly curved, rod-shaped, capsule-forming and stained Gram-negative. Optimal growth was obtained at pH 7.0 (range: pH 5-9) and 30 °C (range: 20-37 °C) in R2A broth. According to the absorption spectrum, carotenoids (455 and 490 nm) and light-harvesting complex LHI (857 nm) were present in the cells. The cells were found to be positive for oxidase and catalase activities. The major respiratory quinone was ubiquinone Q-10. The major fatty acids were identified as C17 : 1ω6c, C16 : 1ω7c/C16 : 1ω6c, C18 : 1ω6c/C18 : 1ω7c and C16 : 0. The major polar lipids were found to consist of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified glycolipid and two sphingoglycolipids. Strain TH057 shared highest 16S rRNA gene sequence similarity to Sandarakinorhabdus limnophila so42 (96.8 %), followed by Polymorphobacter fuscus D40P (95.8 %). The genomic G+C content of strain TH057 was 66.1 mol% based on total genome calculations. The average nucleotide identity and the digital DNA-DNA hybridization value for the complete genomes were 81.0 and 23.0 % between strain TH057 and Sandarakinorhabdus limnophila so42. The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis suggested that strain TH057 represents a novel species within the genus Sandarakinorhabdus, for which the name Sandarakinorhabduscyanobacteriorum sp. nov. is proposed. The type strain is TH057 (=CGMCC 1.15803=LMG 30294).
从中国一个富营养化湖泊的蓝藻聚集体中分离出一株细菌，命名为TH057。观察到细胞略呈弯曲的杆状，形成荚膜，革兰氏染色阴性。在R2A肉汤中，pH 7.0（范围：pH 5 - 9）和30 °C（范围：20 - 37 °C）条件下生长最佳。根据吸收光谱，细胞中存在类胡萝卜素（455和490 nm）和光捕获复合体LHI（857 nm）。发现细胞的氧化酶和过氧化氢酶活性呈阳性。主要呼吸醌为泛醌Q - 10。主要脂肪酸鉴定为C17 : 1ω6c、C16 : 1ω7c/C16 : 1ω6c、C18 : 1ω6c/C18 : 1ω7c和C16 : 0。主要极性脂类由磷脂酰乙醇胺、二磷脂酰甘油、磷脂酰甘油、未鉴定的糖脂和两种鞘糖脂组成。菌株TH057与嗜湖沙拉克诺尔杆菌so42的16S rRNA基因序列相似性最高（96.8 %），其次是暗褐多形杆菌D40P（95.8 %）。基于全基因组计算，菌株TH057的基因组G + C含量为66.1 mol%。菌株TH057与嗜湖沙拉克诺尔杆菌so42完整基因组的平均核苷酸同一性和数字DNA - DNA杂交值分别为81.0和23.0 %。表型、化学分类和系统发育特性以及基因组分析表明，菌株TH057代表沙拉克诺尔杆菌属内的一个新物种，为此提出名称嗜蓝藻沙拉克诺尔杆菌（Sandarakinorhabduscyanobacteriorum）新种。模式菌株为TH057（=CGMCC 1.15803 = LMG 30294）。
