Lu P J, Cheng W L, Huang S C, Chen Y P, Chou H K, Cheng H F
Food and Drug Administration, Ministry of Health and Welfare, No.161-2, Kunyang St, Nangang District, Taipei City, 115-61, Taiwan.
Int J Cosmet Sci. 2015 Dec;37(6):620-6. doi: 10.1111/ics.12239. Epub 2015 Jun 4.
Numerous commercial products contain titanium dioxide (TiO2 ) and zinc oxide (ZnO) nanoparticles (NPs); however, many of these are not labelled as containing NPs. This study sought to develop an effective means of characterizing TiO2 and ZnO NPs in sunscreen sprays, including the size, shape and composition of the particles as well as their aggregation/agglomeration characteristics.
Transmission electron microscopy (TEM) coupled with a window-type microchip K-kit/copper grid and X-ray diffraction (XRD) was used to characterize the oxide NPs.
TME pre-treatment was performed using two approaches: using a conventional copper grid (requiring dilution) and using a K-kit (not requiring dilution). The use of K-kit in conjunction with XRD makes it possible to obtain direct measurements from samples that have not undergone pre-treatment, which could otherwise alter the nature of the samples, such as the degree of agglomeration. XRD was used to obtain information related to particle size and crystal structure. A strong correlation was observed between XRD and TEM measurements.
The proposed measurement methods were shown to be highly effective in the characterization of oxide NPs in sunscreen sprays, providing consistent information related to NPs and their interactions in the formulations.
众多商业产品含有二氧化钛（TiO₂）和氧化锌（ZnO）纳米颗粒（NP）；然而，其中许多产品并未标明含有NP。本研究旨在开发一种有效的方法来表征防晒喷雾中的TiO₂和ZnO NP，包括颗粒的尺寸、形状和组成以及它们的聚集/团聚特性。
采用透射电子显微镜（TEM）结合窗口型微芯片K套件/铜网以及X射线衍射（XRD）来表征氧化物NP。
TEM预处理采用两种方法：使用传统铜网（需要稀释）和使用K套件（不需要稀释）。将K套件与XRD结合使用，可以直接对未经过预处理的样品进行测量，否则可能会改变样品的性质，例如团聚程度。XRD用于获取与颗粒尺寸和晶体结构相关的信息。观察到XRD和TEM测量结果之间有很强的相关性。
所提出的测量方法在表征防晒喷雾中的氧化物NP方面显示出高度有效性，提供了与NP及其在配方中的相互作用相关的一致信息。
